Show simple item record

dc.contributor.author谢黎崖
dc.contributor.author詹传明
dc.contributor.author侯振清
dc.contributor.author陈月
dc.contributor.author吴永良
dc.date.accessioned2016-05-16T09:04:47Z
dc.date.available2016-05-16T09:04:47Z
dc.date.issued2014-3-25
dc.identifier.citation中国现代应用药学,2014,(3):61-64
dc.identifier.issn1007-7693
dc.identifier.otherXDYD201403015
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/98478
dc.description.abstract目的研究双修饰壳聚糖载丝裂霉素C纳米粒在大鼠体内的药动学特征。方法 2组大鼠分别静脉注射4 Mg·kg·1丝裂霉素C纳米粒和丝裂霉素C注射剂后,采用高效液相色谱-串联质谱法(HPlC-MS)测定给药后不同时间点血浆中丝裂霉素C的浓度,计算主要药动学参数。结果丝裂霉素C的线性范围20~1 000μg·l·1,最低定量限为20μg·l·1,提取回收率均>95%,日内、日间精密度rSd均<15%。双修饰壳聚糖载丝裂霉素C纳米粒和丝裂霉素C注射剂T1/2分别为(2.64±0.11)H、(0.49±0.049)H;AuC0-∞分别为(2.01±0.11)Mg·H·1·l·1、(0.93±0.075)Mg·H·1·l·1;Vz分别为(1.52±0.18)l、(0.63±0.065)l;Cl分别为(6.95±0.70)Ml·MIn·1、(15.47±1.89)Ml·MIn·1,2者均有显著性差异。结论该方法灵敏、准确、专一,适用于丝裂霉素C的药动学研究。与丝裂霉素C注射剂相比,双修饰壳聚糖载丝裂霉素C纳米粒具有缓释和长循环的作用。
dc.description.abstractOBJECTIVE To study the pharmacokinetics of dual conjugated chitosan-mitomycin C nanoparticles(CS-MMC-NPs) in rats.METHODS The two groups of rats were injected with CS-MMC-NPs and MMC injection at the dose of 4 mg·kg·1.The concentrations of MMC in plasma at different time were determined by HPLC-MS and the main pharmacokinetic parameters were calculated.RESULTS The calibration curves were linear over the range of 20·1 000 μg·L·1.The limit of quantitation was 20 μg·L·1.The within day and day to day relative standard deviation(RSD) was <15%.The main pharmacokinetic parameters of CS-MMC-NPs and MMC injection were as follows: t1/2 were(2.64±0.11)h and(0.49±0.049)h, AUC0- ∞ were(2.01±0.11)mg·h·1·L·1 and(0.93±0.075)mg·h·1·L·1, Vz were(1.52±0.18)L and(0.63±0.065)L, CL were(6.95±0.70)mL·min·1 and(15.47±1.89)mL·min·1.The differences of parameters were significant between two preparations.CONCLUSION The method is sensitive, accurate, specific for the pharmacokinetic study of CS-MMC-NPs.Compared with MMC injection, CS-MMC-NPs has a controlled releasing rate, a high level of blood concentrations and a long blood circulation time, which benefits the control of acute toxicity of MMC for the rats.
dc.description.sponsorship厦门市科学技术计划项目资助项目(3502Z20114007)
dc.language.isozh_CN
dc.subject丝裂霉素C
dc.subject壳聚糖
dc.subject纳米粒
dc.subject高效液相色谱-质谱联用
dc.subject药动学
dc.subjectmitomycin C
dc.subjectchitosan
dc.subjectnanoparticles
dc.subjectHPLC-MS
dc.subjectpharmacokinetics
dc.title双修饰壳聚糖载丝裂霉素C纳米粒在大鼠体内的药动学研究
dc.title.alternativePharmacokinetics of Dual Conjugated Chitosan-mitomycin C Nanoparticles in Rats
dc.typeArticle


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record