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dc.contributor.authorHuang Rongfu
dc.contributor.author黄荣夫
dc.contributor.authorZhuang Zhixia
dc.contributor.author庄峙厦
dc.contributor.authorYan Qingpi
dc.contributor.author鄢庆枇
dc.contributor.authorLi Jingxi
dc.contributor.author李景喜
dc.contributor.authorChen Shi
dc.contributor.author陈石
dc.contributor.authorWang Xiaoru
dc.contributor.author王小如
dc.date.accessioned2011-06-22T06:53:03Z
dc.date.available2011-06-22T06:53:03Z
dc.date.issued2006
dc.identifier.citationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(10):1411-1414zh_CN
dc.identifier.issn0253-3820
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9724
dc.description.abstractA protein microarray immunoassay method was established in the detection and quantification of E. colt, V. fluvialis and V. parahaemolyticus in water. Cy3-rabbit immunoglobulin G (IgG) was used as a probe. 37 degrees C, 60 min, 0.1 g/L of concentration of IgG. were testified to the optimized conditions in the incubation reaction. For E. colt, the limit of detection was 3.9 x 10(5)cfu/mL, for V. fluvialis was 9.3 x 10(4) efu/mL and for V. parahaemolyticus was 9.9 x 10(4) cfu/mL. Compared with enzyme linked immunosorbent assay (ELISA), the high through-put, rapid and convenient detection of Marine Bacterial Pathogens was demonstrated in the optimized conditions and marine samples were analyzed subsequently using this method effectively.zh_CN
dc.language.isozhzh_CN
dc.publisherSCIENCE CHINA PRESSzh_CN
dc.subjectprotein microarrayzh_CN
dc.subjectfluorescence immunoassayzh_CN
dc.subjectmarine bacterial pathogenzh_CN
dc.titleQuantitative detection of marine pathogenic bacteria using protein microarray immunoassayzh_CN
dc.typeArticlezh_CN


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