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dc.contributor.authorWu, Zhipeng
dc.contributor.authorWang, Sanying
dc.contributor.authorPeng, Xuanxian
dc.contributor.author彭宣宪
dc.contributor.authorChen, Xinhua
dc.contributor.authorYu, Suhong
dc.date.accessioned2011-06-21T15:39:10Z
dc.date.available2011-06-21T15:39:10Z
dc.date.issued2010
dc.identifier.citationFish & Shellfish Immunology,Volume 28, Issue 1, January 2010, Pages 151-158zh_CN
dc.identifier.issn1050-4648
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.fsi.2009.10.015
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9694
dc.description.abstractOutbreaks of infectious diseases in cultured large yellow croaker have resulted in great economic losses. However, information regarding its immune defense is limited. In the present study, an approach by the combination of differential proteomics with EST resource was applied for investigation of a profile of serum immune response by large yellow croaker to Aeromonas hydrophila challenge after immunization and for characterizing of one of the targeted immune molecules. Of the twelve altered proteins involved in the response, eight were identified by MS, in which three were randomly selected for antiserum preparation and were further confirmed by Western blotting. Furthermore, three beta(2)m clones, one of the altered molecules, were obtained from a previously constructed Kidney Smart cDNA library of this fish, and were compared for their identity, which contributed to the identification Of beta(2)m cDNA diversity. Meanwhile, the up-regulated beta(2)m in response to the bacterial immunization and challenge was further confirmed by Western blotting. Our results indicate that beta(2)m is involved in the immune response of large yellow croaker to the challenge by A. hydrophila after immunization, which suggests an efficient approach for characterizing of targeted molecules at both the gene and protein levels. (C) 2009 Elsevier Ltd. All rights reserved.zh_CN
dc.description.sponsorship973 project of China [2006CB101807]; National Natural Science Foundation of China [30871925]; "863" project [2006AA100310]zh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTDzh_CN
dc.subjectLarge yellow croaker Pseudosciaena croceazh_CN
dc.subjectBeta2-microglobulin; Proteomicszh_CN
dc.subjectProteomicszh_CN
dc.subjectExpressed sequence tagzh_CN
dc.subjectAnti-bacterial immune responsezh_CN
dc.subject2-D PAGEzh_CN
dc.titleBeta2-microglobulin is involved in the immune response of large yellow croaker to Aeromonas hydrophila: A proteomic based studyzh_CN
dc.typeArticlezh_CN


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