两种雌雄同体鱼类Gsdf基因的克隆和表达
Cloning and expression patterns of gonadal soma-derived factor (Gsdf) in two hermaphrodite fishes
Abstract
性腺体细胞衍生因子（Gsdf）是转化生长因子β（TGF-β）超家族的新成员。作为一种硬骨鱼特有并仅在性腺表达的生长因子，Gsdf在早期生殖细胞发育中发挥重要作用。雌雄同体鱼类为研究性腺分化和发育机制提供了理想的材料，然而Gsdf在雌雄同体鱼类中的表达模式及功能目前仍不明确。本研究运用组织学、生物化学和分子生物学等技术，对Gsdf在雌性先熟的赤点石斑鱼和雄性先熟的黄鳍鲷两种不同类型雌雄同体鱼类中的表达模式进行了初步研究，主要研究成果如下： 1.观察了赤点石斑鱼和黄鳍鲷性腺发育组织学，根据各级生殖细胞的数量和发育状态以及卵巢和精巢的成熟先后，并结合GSI数据，将赤点石斑鱼性腺发育分为雌性阶段（F... Gonadal soma-derived factor (Gsdf) is a new member of the transforming growth factor beta (TGF-β) superfamily. As an unique growth factor that only expresses in gonads of teleost, Gsdf plays an important role in early germ cell development. Hermaphroditic fishes provide a good experimental model for understanding the mechanisms of sex determination and differentiation. However expression patterns ...