• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Preparation and fluoroimmunoassay application of new red-region fluorescent silica nanoparticles

Thumbnail
Full Text
Preparation and fluoroimmunoassay application of new red-region fluorescent silica nanoparticles.htm (400bytes)
Date
2007
Author
Chen, Xiao-Lan
陈小兰
Zou, Jian-Li
Zhao, Ting-Ting
Li, Zhong-Bao
Collections
  • 化学化工-已发表论文 [14469]
Show full item record
Abstract
This is the first report on the preparation and utilization of a novel red-region fluorescent dye (tetracarboxy aluminum phthalocyanine) doped silica nanoparticles. In these nanoparticles, the tetracarboxy aluminum phthalocyanine molecules were covalently bound to silica matrix to protect the dye leaking from nanoparticles in bio-applications. The surface of the nanoparticles was modified by amino groups and easily bioconjugated with goat anti-human IgG antibody. By employing these nanoparticles as fluorescent probe, a sensitive fluoroimmunoassay method has been developed for the determination of trace level of human IgG. The calibration graph for human IgG was linear over the range of 0-500 ng mL(-1) with a detection limit of 1.6 ng mL(-1). Compared with the corresponding system using free AlC4Pc as a probe for determining human IgG, the sensitivity of the proposed system was notably increased. The method was applied to the analysis of human IgG in human sera with satisfactory results.
Citation
JOURNAL OF FLUORESCENCE,2007,17(2):235-241
URI
http://dx.doi.org/doi:10.1007/s10895-007-0162-8
https://dspace.xmu.edu.cn/handle/2288/9593

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies