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dc.contributor.authorMa, Changxin(Tsinghua Univ, Prot Sci Lab, Minist Educ, Sch Life Sci)
dc.contributor.authorChai, Guoliang(Tsinghua Univ, Prot Sci Lab, Minist Educ, Sch Life Sci)
dc.contributor.authorSu, Hanxia(Tsinghua Univ, Prot Sci Lab, Minist Educ, Sch Life Sci)
dc.contributor.authorZheng, Liang(Tsinghua Univ, Prot Sci Lab, Minist Educ, Sch Life Sci)
dc.contributor.authorWu, Wei(Tsinghua Univ, Prot Sci Lab, Minist Educ, Sch Life Sci)
dc.contributor.authorWang, Xiaoan(Ludong Univ, Sch Life Sci)
dc.contributor.authorYu, Shuizhou
dc.contributor.author于水舟
dc.date.accessioned2011-06-12T06:21:14Z
dc.date.available2011-06-12T06:21:14Z
dc.date.issued2010-09
dc.identifier.citationJournal of Genetics and Genomics,Volume 37, Issue 9,Pages 621-635zh_CN
dc.identifier.issn1673-8527
dc.identifier.urihttp://dx.doi.org/doi:10.1016/S1673-8527(09)60081-3
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9480
dc.description.abstractFreshwater planaria has tremendous capacity to reform the missing part of the body and therefore is considered as one of the most important model organism for regeneration study. At present, Schmidtea mediterranea and Dugesia japonica are the two major species utilized for laboratory manipulations. Dugesia japonica flatworms are widely distributed in the Far East including Cherry Valley region in the north-west area of Beijing, China. We reported here the establishment of an asexual Dugesia japonica strain Pek-1, as a suitable system for regeneration study. Using morphological, karyotypical as well as phylogenetic analyses, we confirmed that these flatworms indeed belonged to Dugesia japonica. We went on to show that the commonly used in situ probes and immunohistochemistry reagents and protocols were applicable to the Pek-1 strain. Using this strain, we carried out small scale analysis on EST, RNAi and gene expression. We identified 193 unique EST sequences and 65 of them had not been reported in planarian. By RNAi analysis, we showed that 48 genes, when down-regulated individually, had no effect on regeneration. Furthermore, we identified 3 groups of tissue specific expressing genes that were useful for cell lineage analysis. We concluded that the Dugesia japonica Pek-1 strain could be another suitable animal model to regeneration research.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30670225]zh_CN
dc.language.isoenzh_CN
dc.publisherSCIENCE CHINA PRESSzh_CN
dc.subjectplanarianzh_CN
dc.subjectDugesia japonicazh_CN
dc.subjectexpression screenzh_CN
dc.titleA small scale expression screen identifies tissue specific markers in the Dugesia japonica strain Pek-1zh_CN
dc.typeArticlezh_CN


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