Efficient expression and primary purification of 6-his tagged human Fas ligand in Dictyostelium discoideum
- 化学化工－已发表论文 
Human Fas ligand (hFasL) is a member of the tumor necrosis factor (TNF) family with many medical interests. To produce this protein efficiently, an improved vector which could express the recombinant hFasL protein with a 6-his tag at its C-terminal was constructed. The new vector was transformed into Dictyostelium discoideum AX3 which then produced 157 mu g hFasL l(-1) stop. Using one-step Ni-affinity chromatography, it was purified with a recovery of 92% and purity of 91%.