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dc.contributor.authorChen, Xihan
dc.contributor.authorZeng, Yonghui
dc.contributor.authorJiao, Nianzhi
dc.contributor.author焦念志
dc.date.accessioned2011-06-11T14:51:02Z
dc.date.available2011-06-11T14:51:02Z
dc.date.issued2006-09-21
dc.identifier.citationMOLECULAR ECOLOGY NOTES,2006,6(4):1278–1281zh_CN
dc.identifier.issn1471-8278
dc.identifier.issnhttp://dx.doi.org/doi:10.1111/j.1471-8286.2006.01514.x
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9463
dc.description.abstractThrough multiple alignment analysis of 16S rDNA sequences from all known genera of Cytophaga-Flavobacteria (CF) cluster, a new primer pair specifically targeting this cluster was developed, greatly facilitating their diversity and function's exploration in marine ecosystems. Compared with previously reported primers, the new primer pair could theoretically retrieve broader CF diversity without decreasing specificity. The effectiveness for field samples was further evaluated by testing the community DNA samples from various marine environments using the optimal polymerase chain reaction (PCR) conditions established in this work. The results showed its robustness and high specificity for amplifying CF cluster's 16S rDNA fragments from complex marine environments.zh_CN
dc.language.isoenzh_CN
dc.publisherBLACKWELL PUBLISHINGzh_CN
dc.subject16S rDNAzh_CN
dc.subjectclone libraryzh_CN
dc.subjectCytophaga-Flavobacteriazh_CN
dc.subjectdiversityzh_CN
dc.subjectmarine environmentszh_CN
dc.subjectprimer designzh_CN
dc.titleDevelopment and evaluation of specific 16S rDNA primers for marine Cytophaga-Flavobacteria clusterzh_CN
dc.typeArticlezh_CN


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