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dc.contributor.authorLu Yinghua
dc.contributor.author卢英华
dc.contributor.authorDeng Xu
dc.contributor.authorCheng Zhijing
dc.contributor.authorLi Qingbiao
dc.contributor.author李清彪
dc.contributor.authorLiu Gang
dc.date.accessioned2011-06-10T15:24:49Z
dc.date.available2011-06-10T15:24:49Z
dc.date.issued2007-05
dc.identifier.citationCHINESE JOURNAL OF CHEMICAL ENGINEERING,2007,15(2):172-177zh_CN
dc.identifier.issn1004-9541
dc.identifier.urihttp://dx.doi.org/doi:10.1016/S1004-9541(07)60054-8
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9453
dc.description.abstractA genetically engineered Escherichia coli JM109 harboring pLF3 was used to produce a hybrid extracellular beta-glucanase. Starting with enzyme production medium, glycerol and yeast extract combined with NaNO3 were screened to be the most suitable carbon and nitrogen source, respectively. Analysis of six components of the enzyme production medium by employing statistical optimization methods such as Plackett-Burman design and steepest ascent showed that yeast extract was the only significant variable and its best concentration for enzyme production was 12g center dot L-1. After optimization of the medium, 297.71U center dot ml(-1) of beta-glucanase activity in the medium and 352350U center dot g(-1) of fl-glucanase selectivity could be obtained, which were 14 and 72 folds higher than those obtained from original medium, respectively. Even higher enzyme activities were achieved by batch cultivations in a conventional stirred bioreactor on the optimized medium.zh_CN
dc.language.isoenzh_CN
dc.publisherCHEMICAL INDUSTRY PRESSzh_CN
dc.subjectbeta-glucanasezh_CN
dc.subjectrecombinant Escherichia colizh_CN
dc.subjectstatistical methodologyzh_CN
dc.subjectmedium optimizationzh_CN
dc.titleEnhanced production of hybrid extracellular beta-glucanase by recombinant Escherichia coli using experimental design methodzh_CN
dc.typeArticlezh_CN


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