• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Enhanced production of hybrid extracellular beta-glucanase by recombinant Escherichia coli using experimental design method

Thumbnail
Full Text
Enhanced production of hybrid extracellular beta-glucanase by recombinant Escherichia coli using experimental design method.htm (413bytes)
Date
2007-05
Author
Lu Yinghua
卢英华
Deng Xu
Cheng Zhijing
Li Qingbiao
李清彪
Liu Gang
Collections
  • 化学化工-已发表论文 [14469]
Show full item record
Abstract
A genetically engineered Escherichia coli JM109 harboring pLF3 was used to produce a hybrid extracellular beta-glucanase. Starting with enzyme production medium, glycerol and yeast extract combined with NaNO3 were screened to be the most suitable carbon and nitrogen source, respectively. Analysis of six components of the enzyme production medium by employing statistical optimization methods such as Plackett-Burman design and steepest ascent showed that yeast extract was the only significant variable and its best concentration for enzyme production was 12g center dot L-1. After optimization of the medium, 297.71U center dot ml(-1) of beta-glucanase activity in the medium and 352350U center dot g(-1) of fl-glucanase selectivity could be obtained, which were 14 and 72 folds higher than those obtained from original medium, respectively. Even higher enzyme activities were achieved by batch cultivations in a conventional stirred bioreactor on the optimized medium.
Citation
CHINESE JOURNAL OF CHEMICAL ENGINEERING,2007,15(2):172-177
URI
http://dx.doi.org/doi:10.1016/S1004-9541(07)60054-8
https://dspace.xmu.edu.cn/handle/2288/9453

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies