A new method for high-resolution NMR spectra in inhomogeneous fields with efficient solvent suppression

Abstract

High-resolution nuclear magnetic resonance (NMR) is one of the most powerful tools for analyzing molecular structures and dynamics. Magnetic field homogeneity is required for conventional high-resolution spectra. However, there are many chemical and/or biological circumstances where the spatial homogeneities of the magnetic fields are degraded. Intense solvent signal is another obstacle for obtaining high-resolution spectra, especially in in vivo and in situ NMR spectroscopy. In this paper, a new pulse sequence based on intermolecular multiple quantum coherence (iMQC) was reported. This sequence can effectively remove the effect of magnetic field inhomogeneity and suppress the solvent signal. It can recover the spectral information such as chemical shifts, coupling constants, multiplet patterns, and relative peak areas in inhomogeneous fields. Theoretical analyses and experimental verifications are presented to demonstrate the feasibility of this method.