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dc.contributor.authorZhao, Honghaizh_CN
dc.contributor.authorZhang, Tongenzh_CN
dc.contributor.authorXia, Chunzh_CN
dc.contributor.authorShi, Leizh_CN
dc.contributor.authorWang, Shaojiezh_CN
dc.contributor.authorZheng, Xinpengzh_CN
dc.contributor.authorHu, Tianhuizh_CN
dc.contributor.authorZhang, Bingzh_CN
dc.contributor.author夏春zh_CN
dc.contributor.author胡天惠zh_CN
dc.contributor.author张兵zh_CN
dc.date.accessioned2015-07-22T07:37:26Z
dc.date.available2015-07-22T07:37:26Z
dc.date.issued2014 FEBzh_CN
dc.identifier.citationJOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2014,18(2):283-292zh_CN
dc.identifier.otherWOS:000330190200010zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93683
dc.descriptionNational Natural Science Foundation of China [81072015, 81371952]; Natural Science Foundation of Fujian, China [2010D007]; Medical Innovation Foundation of Fujian, China [2011-CXB-36]; Natural Science Foundation of Xiamen, China [3502Z20124046]; Xiamen Science and Technology Key program grant, Fujian, Chinazh_CN
dc.description.abstractBerberine, a plant alkaloid used in Chinese medicine, has broad cell-protective functions in a variety of cell lines. Chondrocyte apoptosis contributes to the pathogenesis of cartilage degeneration in osteoarthritis (OA). However, little is known about the effect and underlying mechanism of berberine on OA chondrocytes. Here, we assessed the effects of berberine on cartilage degeneration in interleukin-1 (IL-1)-stimulated rat chondrocytes and in a rat model of OA. The results of an MTT assay and western blotting analysis showed that berberine attenuated the inhibitory effect of IL-1 on the cell viability and proliferating cell nuclear antigen expression in rat chondrocytes. Furthermore, berberine activated Akt, which triggered p70S6K/S6 pathway and up-regulated the levels of aggrecan and Col II expression in IL-1-stimulated rat chondrocytes. In addition, berberine increased the level of proteoglycans in cartilage matrix and the thickness of articular cartilage, with the elevated levels of Col II, p-Akt and p-S6 expression in a rat OA model, as demonstrated by histopathological and immunohistochemistry techniques. The data thus strongly suggest that berberine may ameliorate cartilage degeneration from OA by promoting cell survival and matrix production of chondrocytes, which was partly attributed to the activation of Akt in IL-1-stimulated articular chondrocytes and in a rat OA model. The resultant chondroprotective effects indicate that berberine merits consideration as a therapeutic agent in OA.zh_CN
dc.language.isoen_USzh_CN
dc.publisherWILEY-BLACKWELLzh_CN
dc.source.urihttp://dx.doi.org/10.1111/jcmm.12186zh_CN
dc.subjectCRUCIATE LIGAMENT TRANSECTIONzh_CN
dc.subjectARTICULAR CHONDROCYTESzh_CN
dc.subjectPROTEOGLYCAN SYNTHESISzh_CN
dc.subjectPROTEIN-KINASEzh_CN
dc.subjectCANCER CELLSzh_CN
dc.subjectAPOPTOSISzh_CN
dc.subjectEXPRESSIONzh_CN
dc.subjectPATHWAYzh_CN
dc.subjectDEATHzh_CN
dc.subjectPHOSPHORYLATIONzh_CN
dc.titleBerberine ameliorates cartilage degeneration in interleukin-1 beta-stimulated rat chondrocytes and in a rat model of osteoarthritis via Akt signallingzh_CN
dc.typeArticlezh_CN


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