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dc.contributor.authorYang, Xiao-Ningzh_CN
dc.contributor.authorLu, Ya-Pizh_CN
dc.contributor.authorLiu, Jing-Jingzh_CN
dc.contributor.authorHuang, Jian-Kunzh_CN
dc.contributor.authorLiu, Yun-Pengzh_CN
dc.contributor.authorXiao, Chuan-Xingzh_CN
dc.contributor.authorJazag, Amarsanaazh_CN
dc.contributor.authorRen, Jian-Linzh_CN
dc.contributor.authorGuleng, Bayasizh_CN
dc.contributor.author任建林zh_CN
dc.contributor.author巴亚斯古楞zh_CN
dc.date.accessioned2015-07-22T07:37:16Z
dc.date.available2015-07-22T07:37:16Z
dc.date.issued2014 JULzh_CN
dc.identifier.citationDIGESTIVE DISEASES AND SCIENCES, 2014,59(7):1428-1435zh_CN
dc.identifier.otherWOS:000338344500016zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93592
dc.descriptionNational Natural Science Foundation of China [81370505, 81370591, 91229201]; Key Projects for Technology Plan of Xiamen [3502Z20100002]; Ministry of Health Foundation for State Key Clinical Department in Chinazh_CN
dc.description.abstractTrefoil factor family 1 (TFF1) is a member of the TFF-domain peptide family involved in epithelial restitution and cell motility. Recently, we screened Piezo1 as a candidate TFF1-binding protein. We aimed to confirm Piezo1 as a novel TFF1 binding protein and to assess the role of this interaction in mediating gastric cancer cell mobility. This interaction was confirmed by co-immunoprecipitation and co-localisation of TFF1 and Piezo1 in GES-1 cells. We used stable RNA interference to knockdown Piezo1 protein expression and restored the expression of TFF1 in the gastric cancer cell lines SGC-7901 and BGC-823. Cell motility was evaluated using invasion assay and migration assay in vitro. The expression levels of the integrin subunits beta 1, beta 5, alpha 1 as well as the expression of beta-catenin and E-cadherin were detected by Western blot. We demonstrate that TFF1, but not TFF2 or TFF3, bind to and co-localize with Piezo1 in the cytoplasm in vitro. TFF1 interacts with the C-terminal portion of the Piezo1 protein. Wound healing and trans-well assays demonstrated that the restored expression of TFF1 promoted cell mobility in gastric cancer cells, and this effect was attenuated by the knockdown of Piezo1. Western blots demonstrated the decreased expression of integrin beta 1 in Piezo1-knockdown cells. Our data demonstrate that Piezo1 is a novel TFF1 binding protein that is important for TFF1-mediated cell migration and suggest that this interaction may be a therapeutic target in the invasion and metastasis of gastric cancer.zh_CN
dc.language.isoen_USzh_CN
dc.publisherSPRINGERzh_CN
dc.source.urihttp://dx.doi.org/10.1007/s10620-014-3044-3zh_CN
dc.subjectSIGNALING PATHWAYSzh_CN
dc.subjectBETA-CATENINzh_CN
dc.subjectGROWTHzh_CN
dc.subjectACTIVATIONzh_CN
dc.subjectEXPRESSIONzh_CN
dc.subjectPEPTIDESzh_CN
dc.subjectRECEPTORzh_CN
dc.subjectGENEzh_CN
dc.subjectINTEGRINSzh_CN
dc.subjectMIGRATIONzh_CN
dc.titlePiezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitrozh_CN
dc.typeArticlezh_CN


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