Lipoxin A(4) regulates expression of the estrogen receptor and inhibits 17 beta-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells
- 医学院－已发表论文 
Objective: To study the role of lipoxin A(4) (LXA(4)) in endometriosis. Design: Molecular analysis in human samples and primary human endometriotic stromal cells (ESCs). Setting: University hospital. Patient(s): Forty-nine premenopausal women (30 patients with endometriosis and 19 controls). Intervention(s): Normal and ectopic endometrial biopsies obtained during surgery performed during the proliferative phase of the menstrual cycle; ESCs used for in vitro studies. Main Outcome Measure(s): Levels of LXA(4) measured by enzyme-linked immunosorbent assay (ELISA); mRNA levels of the estrogen receptor (ER), progestogen receptor (PR), tumor necrosis factor alpha (TNF-alpha), and interleukin 6 (IL-6) quantified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR); and p38 mitogen-activated protein kinase (p38 MAPK) phosphorylation evaluated by Western blotting. Result(s): The LXA(4) expression level decreased in ectopic tissue as well as ER alpha and PR, although the expression of ER beta increased in ectopic endometrium compared with the controls. Investigations with correlation analysis revealed the expression of LXA(4) was positively correlated with ER alpha and negatively correlated with ER beta in vivo. Moreover, administering LXA(4) could augment ER beta expression in ESCs and inhibit the 17 beta-estradiol-induced phosphorylation of p38 MAPK very likely through ER beta. Conclusion(s): Our findings indicate that LXA(4) regulates ER beta expression and inhibits 17 beta-estradiol-induced phosphorylation of p38 MAPK, very likely through ER beta in ESCs. (C)2014 by American Society for Reproductive Medicine.