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dc.contributor.authorCao, Luzh_CN
dc.contributor.authorZhou, Yanmingzh_CN
dc.contributor.authorZhai, Beibeizh_CN
dc.contributor.authorLiao, Jianzh_CN
dc.contributor.authorXu, Wenzh_CN
dc.contributor.authorZhang, Ruixiuzh_CN
dc.contributor.authorLi, Jingzh_CN
dc.contributor.authorZhang, Yuzh_CN
dc.contributor.authorChen, Leizh_CN
dc.contributor.authorQian, Haihuazh_CN
dc.contributor.authorWu, Mengchaozh_CN
dc.contributor.authorYin, Zhengfengzh_CN
dc.contributor.author周彦明zh_CN
dc.date.accessioned2015-07-22T07:36:44Z
dc.date.available2015-07-22T07:36:44Z
dc.date.issued2011-06-14zh_CN
dc.identifier.citationBMC GASTROENTEROLOGY, 2011,11zh_CN
dc.identifier.otherWOS:000292736800001zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93293
dc.descriptionNational High-Tech Research and Development Program of China [2007AA02Z461]; China National Key Projects for Infectious Disease [2008ZX10002-021]; National Natural Science Foundation of China [30672002, 30801342]zh_CN
dc.description.abstractBackground: Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs. Methods: Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs. Results: The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability. Conclusions: Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.zh_CN
dc.language.isoen_USzh_CN
dc.publisherBMC GASTROENTEROLzh_CN
dc.source.urihttp://dx.doi.org/10.1186/1471-230X-11-71zh_CN
dc.subjectIN-VITRO PROPAGATIONzh_CN
dc.subjectHUMAN LIVER-CANCERzh_CN
dc.subjectHEPATOCELLULAR-CARCINOMAzh_CN
dc.subjectSTEM/PROGENITOR CELLSzh_CN
dc.subjectIDENTIFICATIONzh_CN
dc.subjectPOPULATIONzh_CN
dc.subjectNOTCHzh_CN
dc.subjectCARCINOGENESISzh_CN
dc.subjectESTABLISHMENTzh_CN
dc.subjectACTIVATIONzh_CN
dc.titleSphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lineszh_CN
dc.typeArticlezh_CN


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