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dc.contributor.author林元烧
dc.contributor.author曹文清
dc.contributor.author方旅平
dc.contributor.author刘茜茜
dc.contributor.author李少菁
dc.date.accessioned2011-06-02T01:08:46Z
dc.date.available2011-06-02T01:08:46Z
dc.date.issued2004-11
dc.identifier.citation厦门大学学报(自然科学版),2004(6):842-846zh_CN
dc.identifier.issn0438-0479
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9322
dc.description.abstract【中文摘要】 采用苯酚-氯仿提取、异丙醇沉淀提取中华假磷虾基因组DNA;以相应引物经PCR扩增得到线粒体DNACOI片段;PCR产物采用化学法与载体(pGEM-TEasyVector)重组基因、热休克法转化重组质粒至感受态大肠杆菌(JM109)、氨苄LB培养基扩大培养;测序.结果表明,中华假磷虾线粒体COI碱基709bp,其碱基组成A、T、G、C含量分别为28 59%、35 35%、17 61%和18 45%(国际基因库索引号AY754819);与同科内其它3属10种磷虾的mtCOI基因片段核苷酸组成相似. 【英文摘要】 Pseudeuphausia sinica (Euphausiacea, Crustacea) is abundant in the coastal and neritic waters of the East China Sea and the Southern Yellow Sea all the year round, and is the important food for many fishes, especially for fish larvae. The genomic DNA of Pseudeupharsia sinica, collected from Xiamen waters in May of 2002, was obtained by phenol: Chloroform:isoamyl alcohol (25:24:1) extraction and isopropyl alcohol precipitation. Mitochondrial DNA COI gene was successfully amplified and sequenced. 709 base pai...zh_CN
dc.description.sponsorship国家重点基础研究发展规划项目(973)(G1990437-08)资助zh_CN
dc.language.isozhzh_CN
dc.publisher《厦门大学学报(自然科学版)》编辑部zh_CN
dc.subject中华假磷虾zh_CN
dc.subject线粒体DNAzh_CN
dc.subjectmtCOIzh_CN
dc.subject厦门zh_CN
dc.subjectPseudeuphausia sinicazh_CN
dc.subjectmitochondrial DNAzh_CN
dc.subjectXiamenzh_CN
dc.title中华假磷虾线粒体DNA COI基因片段序列分析zh_CN
dc.title.alternativeAnalysis of Mitochondrial DNA CO I Gene Fragment of Pseudeuphausia sinicazh_CN
dc.typeArticlezh_CN


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