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dc.contributor.authorZhang, Ke-jiezh_CN
dc.contributor.authorLu, Quan-yizh_CN
dc.contributor.authorNiu, Xiao-qingzh_CN
dc.contributor.authorZhang, Pengzh_CN
dc.contributor.authorZhao, Jiang-ningzh_CN
dc.contributor.authorWang, Zhaozh_CN
dc.contributor.authorHu, Jia-shengzh_CN
dc.contributor.authorLi, Puzh_CN
dc.contributor.authorLiu, Wen-lizh_CN
dc.contributor.author张可杰zh_CN
dc.contributor.author鹿全意zh_CN
dc.contributor.author牛小青zh_CN
dc.contributor.author张鹏zh_CN
dc.contributor.author赵江宁zh_CN
dc.contributor.author王昭zh_CN
dc.contributor.author胡嘉升zh_CN
dc.date.accessioned2015-07-22T07:36:28Z
dc.date.available2015-07-22T07:36:28Z
dc.date.issued2009-02zh_CN
dc.identifier.citationACTA PHARMACOLOGICA SINICA, 2009,30(2):153-158zh_CN
dc.identifier.otherWOS:000263416100002zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93154
dc.descriptionNational Natural Science Foundation of China [30570773]zh_CN
dc.description.abstractAim: To investigate the role of the Notch1 signaling pathway in growth arrest of an esophageal carcinoma cell line (EC109) in vitro and the mechanism involved. Methods: An intracellular domain of Notch1 (ICN) was transfected into cultured EC109 cells by lipofectamine transfection. Subsequently, the proliferation of the transfected cells was measured by an MTT assay. Cell cycle distribution was analyzed by flow cytometry. Human papillomavirus type 18 (HPV18) E6/E7 mRNA expression was detected by RT-PCR, and p53 protein expression was detected by Western blot. Results: Activation of Notch1 signaling resulted in inhibition of EC109 cell proliferation with the induction of G2/M arrest, downmodulation of HPV18 E6/E7 gene expression, and upregulation of p53 expression. Conclusion: Repression of HPV18 E6/E7 expression by Notch1 signaling results in the activation of p53-mediated pathways with concomitant growth suppression of HPV18-positive EC109 cells.zh_CN
dc.language.isoen_USzh_CN
dc.publisherACTA PHARMACOL SINzh_CN
dc.source.urihttp://dx.doi.org/10.1038/aps.2008.16zh_CN
dc.subjectCANCER CELLSzh_CN
dc.subjectP53zh_CN
dc.subjectDIFFERENTIATIONzh_CN
dc.subjectHEMATOPOIESISzh_CN
dc.subjectACTIVATIONzh_CN
dc.subjectARRESTzh_CN
dc.subjectROLESzh_CN
dc.titleNotch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expressionzh_CN
dc.typeArticlezh_CN


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