Show simple item record

dc.contributor.authorChen, X.zh_CN
dc.contributor.authorZhang, L.zh_CN
dc.contributor.authorQi, Z.zh_CN
dc.contributor.authorGuo, B.zh_CN
dc.contributor.authorZhong, L.zh_CN
dc.contributor.authorShen, B.zh_CN
dc.contributor.authorYan, Z.zh_CN
dc.contributor.authorZhang, J.zh_CN
dc.contributor.author陈珣zh_CN
dc.contributor.author张玲zh_CN
dc.contributor.author戚智zh_CN
dc.contributor.author张杰zh_CN
dc.date.accessioned2015-07-22T07:36:27Z
dc.date.available2015-07-22T07:36:27Z
dc.date.issued2009-12zh_CN
dc.identifier.citationTRANSPLANTATION PROCEEDINGS, 2009,41(10):4307-4312zh_CN
dc.identifier.otherWOS:000272968800055zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93142
dc.descriptionNational Natural Science Foundation of China [30600606]; Second Affiliated Hospital, Harbin Medical University [BS2008-37]zh_CN
dc.description.abstractThe sulfated glucomannan can be used to filter the heparin-binding properties of cytokines. In this study, novel sulfated glucomannan-barium-alginate (SGA) microcapsules were prepared to encapsulate islets with barium-alginate (ABa) and calcium alginate-poly-L-lysine (APA) microcapsules as controls. SD rat islets were purified as donor cells to Lewis rats that had been treated with streptozotocin. Intraperitoneal transplantation was performed with about 3000 islet equivalent (IEQ) rat. At week three after transplantation, the concentrations of monocyte chemotactic protein-1 (MCP-1), interleukin (IL)-1 beta, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha in intraperitoneal fluid were determined using ELISA. At week 8, the islet cell mass in the abdominal microcapsules was excised to test insulin release. The EB-FDA fluorescence staining method was used to observe the functional activity of the islet cells. Compared with ABa and APA microcapsules, SGA microcapsules showed significantly decreased MCP-1 secretion by beta-cells. Also, the concentrations of cytokines IL-1 beta, IFN-gamma, and TNF-alpha were decreased significantly. The activity of the transplanted islets was significantly improved in SGA microcapsules, which shielded against cytokines better than ABa or APA microcapsules and may serve as novel method.zh_CN
dc.language.isoen_USzh_CN
dc.publisherTRANSPL Pzh_CN
dc.source.urihttp://dx.doi.org/10.1016/j.transproceed.2009.09.070zh_CN
dc.subjectPANCREATIC-ISLETSzh_CN
dc.subjectSURVIVALzh_CN
dc.subjectINSULINzh_CN
dc.titleNovel Sulfated Glucomannan-Barium-Alginate Microcapsules in Islet Transplantation: Significantly Decreased the Secretion of Monocyte Chemotactic Protein 1 and Improved the Activity of Islet in Ratszh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record