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dc.contributor.authorGong, Taiqianzh_CN
dc.contributor.authorXue, Zengfuzh_CN
dc.contributor.authorTang, Shanhongzh_CN
dc.contributor.authorZheng, Xiushanzh_CN
dc.contributor.authorXu, Guanghuizh_CN
dc.contributor.authorGao, Liucunzh_CN
dc.contributor.authorZhao, Guohongzh_CN
dc.contributor.authorHong, Liuzh_CN
dc.contributor.authorTang, Guangbozh_CN
dc.contributor.authorZhang, Hongweizh_CN
dc.contributor.authorWang, Ruwenzh_CN
dc.contributor.authorJiang, Yaoguangzh_CN
dc.contributor.authorFan, Daimingzh_CN
dc.contributor.author薛增福zh_CN
dc.date.accessioned2015-07-22T07:36:21Z
dc.date.available2015-07-22T07:36:21Z
dc.date.issued2012-06zh_CN
dc.identifier.citationCANCER BIOLOGY & THERAPY, 2012,13(8):606-613zh_CN
dc.identifier.otherWOS:000305333500006zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93108
dc.descriptionChina Postdoctoral Science Foundation [20090461447]; National Natural Science Foundation of China [81172288]zh_CN
dc.description.abstractTwist-1 protein (also called Twist) has been suggested to be involved in tumor epithelial-mesenchymal transition (EMT) related progression, however, the mechanism by which Twist promotes lymph node metastasis is not fully understood. In the present study, we found that nuclear Twist expression is clearly correlated with lymph node (LN) metastasis as determined by immunohistochemistry (IHC). A highly invasive EC109 cell subline, EC109-P, was established by repeated in vitro transwell isolations for the cell model. Immunofluorescence (IF) assay demonstrated that nuclear Twist expression was markedly higher in the highly invasive EC109-P cell line when compared with EC109 and EC9706 cells. Based on our cell model, the function and mechanism by which Twist regulates LN metastasis in ESCC was investigated. The results showed that the overexpression of Twist could significantly increase the invasion and VEGF-C expression of EC9706 cells, whereas the knockdown of Twist expression results in the opposite effects. This finding was further strengthened by the results of the analysis of co-expression of Twist and VEGF-C by IHC in ESCC clinical samples. In summary, our study indicates that nuclear Twist plays an important role in ESCC lymphatic metastasis by increasing the expression of VEGF-C. The combination of Twist and VEGF-C detection could be a reliable prediction of LN metastasis in ESCC.zh_CN
dc.language.isoen_USzh_CN
dc.publisherCANCER BIOL THERzh_CN
dc.source.urihttp://dx.doi.org/10.4161/cbt.19851zh_CN
dc.subjectGROWTH-FACTOR-Czh_CN
dc.subjectEPITHELIAL-MESENCHYMAL TRANSITIONzh_CN
dc.subjectVEGF-Czh_CN
dc.subjectBREAST-CANCERzh_CN
dc.subjectCOLORECTAL-CANCERzh_CN
dc.subjectOVEREXPRESSIONzh_CN
dc.subjectINDUCTIONzh_CN
dc.subjectINVASIONzh_CN
dc.subjectLYMPHANGIOGENESISzh_CN
dc.subjectMIGRATIONzh_CN
dc.titleNuclear expression of Twist promotes lymphatic metastasis in esophageal squamous cell carcinomazh_CN
dc.typeArticlezh_CN


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