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dc.contributor.authorXiao, Xinyezh_CN
dc.contributor.authorHe, Huanzh_CN
dc.contributor.authorLin, Zhirongzh_CN
dc.contributor.authorLuo, Pingpingzh_CN
dc.contributor.authorHe, Huizh_CN
dc.contributor.authorZhou, Tongzh_CN
dc.contributor.authorZhou, Yuepingzh_CN
dc.contributor.authorLiu, Zuguozh_CN
dc.contributor.author刘祖国zh_CN
dc.date.accessioned2015-07-22T07:36:20Z
dc.date.available2015-07-22T07:36:20Z
dc.date.issued2012-01zh_CN
dc.identifier.citationINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2012,53(1):191-197zh_CN
dc.identifier.otherWOS:000302694500031zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93099
dc.descriptionNational Basic Research Program of China (Project 973) [2011CB504606]; National Natural Science Foundation of China [30931160432, 30872810]; Technological innovation platform program of Fujian Province, China [2009J1013]; Cross-strait technological platform of Chinese medicine [3502Z20100006]; Watsin Company (Shenzhen, China)zh_CN
dc.description.abstractPURPOSE. To investigate the therapeutic effects and possible mechanisms of epidermal growth factor (EGF) on the mouse dry eye model induced by benzalkonium chloride (BAC). METHODS. The eye drop containing EGF was topically administered (3 ng per day) on a BAC-induced dry eye model. The following clinical indications of dry eye were evaluated on Days 2, 4, and 6: tear break-up time (BUT), corneal fluorescein staining, inflammatory index, and tear volume. Global specimens were collected on Day 6 and then the following examinations were performed: histologic investigation, TUNEL assay to measure the dead cells, periodic acid-schiff (PAS) assay to detect goblet cells, and immunostaining of antibodies of Ki-67, EGF receptor (EGFR), and MUC1 in the corneas. The levels of EGFR and p-ERK of the corneas were also measured by Western blot analysis. RESULTS. EGF resulted in longer BUTs on Days 2 and 6, lower fluorescein staining scores on Days 4 and 6, while no significant changes in inflammatory index or tear volume. EGF induced higher EGFR expression in corneal tissues by immunofluorescent staining and Western blot analysis. EGF also upregulated p-ERK, increased Ki-67 positive cells, and decreased TUNEL positive cells. In addition, EGF significantly increased the goblet cells number and MUC1 expression in the epithelium. CONCLUSIONS. Topical application of EGF presented clinical improvements on dry eye by stabilizing the tear film and maintaining the integrity of epithelium. The results indicate that EGF has potential as a therapeutic agent in clinical treatment of dry eye. (Invest Ophthalmol Vis Sci. 2012;53:191-197) DOI:10.1167/iovs.11-8553zh_CN
dc.language.isoen_USzh_CN
dc.publisherINVEST OPHTH VIS SCIzh_CN
dc.source.urihttp://dx.doi.org/10.1167/iovs.11-8553zh_CN
dc.subjectGOBLET CELL-DENSITYzh_CN
dc.subjectOCULAR SURFACEzh_CN
dc.subjectFACTOR RECEPTORzh_CN
dc.subjectAUTOLOGOUS-SERUMzh_CN
dc.subjectTEARzh_CN
dc.subjectPREVALENCEzh_CN
dc.subjectEXPRESSIONzh_CN
dc.subjectRABBITzh_CN
dc.subjectEGFzh_CN
dc.subjectCYCLOSPORINEzh_CN
dc.titleTherapeutic Effects of Epidermal Growth Factor on Benzalkonium Chloride-Induced Dry Eye in a Mouse Modelzh_CN
dc.typeArticlezh_CN


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