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dc.contributor.authorLiu, Ying-Fuzh_CN
dc.contributor.authorChen, Yong-Hengzh_CN
dc.contributor.authorLi, Mao-Yuzh_CN
dc.contributor.authorZhang, Peng-Feizh_CN
dc.contributor.authorPeng, Fangzh_CN
dc.contributor.authorLi, Guo-Qingzh_CN
dc.contributor.authorXiao, Zhi-Qiangzh_CN
dc.contributor.authorChen, Zhu-Chuzh_CN
dc.contributor.author刘迎福zh_CN
dc.date.accessioned2015-07-22T07:36:19Z
dc.date.available2015-07-22T07:36:19Z
dc.date.issued2012-03zh_CN
dc.identifier.citationMEDICAL ONCOLOGY, 2012,29(1):174-184zh_CN
dc.identifier.otherWOS:000300317200027zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93097
dc.descriptionNational Key Basic Research Program of China [2001CB510207]; Fundamental Research Funds for the Central Universities [2010121104]; Ministry of Education of China [2002-48]; Science and Technology Committee of Hunan, China [04XK1001-1, 05SK1004-1]; Public Health Bureau of Hunan Province, China [Z02-04]zh_CN
dc.description.abstractLymph node status is a strong predictor of outcome for lung adenocarcinoma (AdC) patients. To explore novel potential protein markers for predicting lymph node metastasis of lung AdC, differential proteomic analysis on microdissected cancer cells from primary lung AdC and matched lymph node (LN) metastatic tissues by laser capture microdissection (LCM) was conducted using two-dimensional differential in-gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS). Annexins including annexin-1, annexin-2 and annexin-3 were identified and found to be overexpressed in matched LN metastatic tissues compared to primary lung AdC. Furthermore, differential expression levels of the three annexins were evaluated in paraffin-embedded 188 primary lung AdC tissues and 65 matched positive lymph node specimens using immunohistochemistry. High expression of annexin-1, annexin-2, and annexin-3 was all frequently observed in matched positive lymph node tissues compared to primary lung AdC. In primary lung AdC, expression levels of the three annexins in primary lymph node-positive AdC tissues were higher than primary lymph node-negative AdC tissues. Multivariate logistic regression analysis indicated annexin-1, annexin-2, and annexin-3 were all significant risk factors for lymph node metastasis. Furthermore, statistical analysis indicated that the concomitant expression of annexin-1/annexin-2, annexin-1/annexin-3, or annexin-2/annexin-3 and combined expression of all three markers had stronger correlation with lymph node metastasis. Our results suggest that annexin-1, annexin-2, and annexin-3 are identified as potential biomarkers associated with lymph node metastasis in lung AdC.zh_CN
dc.language.isoen_USzh_CN
dc.publisherMED ONCOLzh_CN
dc.source.urihttp://dx.doi.org/10.1007/s12032-010-9761-3zh_CN
dc.subjectLASER CAPTURE MICRODISSECTIONzh_CN
dc.subjectDIFFERENCE GEL-ELECTROPHORESISzh_CN
dc.subjectCANCER CELL-LINEzh_CN
dc.subjectNASOPHARYNGEAL CARCINOMAzh_CN
dc.subjectCOLORECTAL-CARCINOMAzh_CN
dc.subjectSQUAMOUS CARCINOMAzh_CN
dc.subjectEXPRESSIONzh_CN
dc.subjectIDENTIFICATIONzh_CN
dc.subjectBIOMARKERSzh_CN
dc.subjectINVASIONzh_CN
dc.titleQuantitative proteomic analysis identifying three annexins as lymph node metastasis-related proteins in lung adenocarcinomazh_CN
dc.typeArticlezh_CN


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