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dc.contributor.authorLi, Manxiangzh_CN
dc.contributor.authorLiu, Yuanzh_CN
dc.contributor.authorJin, Faguangzh_CN
dc.contributor.authorSun, Xiuzhenzh_CN
dc.contributor.authorLi, Zongfangzh_CN
dc.contributor.authorLiu, Yunzh_CN
dc.contributor.authorFang, Pingzh_CN
dc.contributor.authorShi, Hongyangzh_CN
dc.contributor.authorJiang, Xingtangzh_CN
dc.contributor.author江兴堂zh_CN
dc.date.accessioned2015-07-22T07:36:18Z
dc.date.available2015-07-22T07:36:18Z
dc.date.issued2012-11-02zh_CN
dc.identifier.citationFEBS LETTERS, 2012,586(21):3888-3893zh_CN
dc.identifier.otherWOS:000310529000020zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93091
dc.descriptionChinese National Science Foundation [81070045, 8117051]; Second Affiliated Hospital of Medical College of Xi'an Jiaotong University, PR Chinazh_CN
dc.description.abstractEndothelin-1 (ET-1) dose-dependently increased HIF1 alpha expression in pulmonary artery smooth muscle cells (PASMCs). Inhibition of protein synthesis did not affect ET-1-induced HIF1 alpha expression. The maximum effect of ET-1 was similar to that caused by proteasome inhibitor MG132. Further study indicates that ET-1 also dose-dependently stimulated calcineurin activation, specific calcineurin inhibitor cyclosporine A (CsA), abolished ET-1-induced HIF1 alpha elevation, and reversed ET-1-induced RACK1 (receptor of activated protein kinase C 1) de-phosphorylation. Endothelin receptor A was found to specifically mediate the effects of ET-1. To examine whether RACK1 is particularly involved in proteasome-dependent HIF1 alpha degradation, RACK1 was silenced by siRNA transfection. Cells lacking RACK1 exhibited significant elevation of HIF1 alpha protein level. Taken together, our study suggests that ET-1 suppressed proteasome-dependent HIF1 alpha degradation by calcineurin-dependent RACK1 de-phosphorylation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.zh_CN
dc.language.isoen_USzh_CN
dc.publisherFEBS LETTzh_CN
dc.source.urihttp://dx.doi.org/10.1016/j.febslet.2012.08.036zh_CN
dc.subjectINTERMITTENT HYPOXIAzh_CN
dc.subjectINTRACELLULAR CA2+zh_CN
dc.subjectHYPERTENSIONzh_CN
dc.subjectACTIVATIONzh_CN
dc.subjectDEGRADATIONzh_CN
dc.subjectHIF-1-ALPHAzh_CN
dc.subjectINHIBITIONzh_CN
dc.subjectRACK1zh_CN
dc.subjectMANAGEMENTzh_CN
dc.subjectHSP90zh_CN
dc.titleEndothelin-1 induces hypoxia inducible factor 1 alpha expression in pulmonary artery smooth muscle cellszh_CN
dc.typeArticlezh_CN


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