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Multianalyte immunoassay based on surface-enhanced Raman spectroscopy

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Multianalyte immunoassay based on surface-enhanced Raman spectroscopy.htm (407bytes)
Date
2007-03
Author
Cui, Yan
Ren, Bin
任斌
Yao, Jian-Lin
Gu, Ren-Ao
Tian, Zhong-Qun
田中群
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  • 化学化工-已发表论文 [14469]
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Abstract
In this paper, two immunoassay methods based on SERS are developed for multiplex analysis, both of which stemmed from the concept of forming a sandwich structure 'capture antibody substrate/antigen/Raman-reporter-labeled immuno-nanoparticles'. They are two-molecule labeled one-nanoparticle and one-molecule labeled two-nanoparticle methods. In both the methods, two different antibodies covalently bound to a solid substrate can specifically capture two different antigens from a sample. The captured antigens in turn bind selectively to their corresponding antibodies immobilized on Raman-reporter-labeled nanoparticles. Multianalyte immunoassay is successfully demonstrated by the detection of characteristic Raman bands of the probe molecules only when the antigen and antibody are matched. Copyright (C) 2007 John Wiley & Sons, Ltd.
Citation
JOURNAL OF RAMAN SPECTROSCOPY,2007,38(7):896-902
URI
http://dx.doi.org/doi:10.1002/jrs.1742
https://dspace.xmu.edu.cn/handle/2288/9307

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