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dc.contributor.authorLaufman, Orlyzh_CN
dc.contributor.authorHong, WanJinzh_CN
dc.contributor.authorLev, Simazh_CN
dc.contributor.author洪万进zh_CN
dc.date.accessioned2015-07-22T07:35:03Z
dc.date.available2015-07-22T07:35:03Z
dc.date.issued2011-08-08zh_CN
dc.identifier.citationJOURNAL OF CELL BIOLOGY, 2011,194(3):459-472zh_CN
dc.identifier.otherWOS:000293693500011zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/93063
dc.descriptionFederal German Ministry for Education and Researchzh_CN
dc.description.abstractThe conserved oligomeric Golgi (COG) complex has been implicated in the regulation of endosome to trans-Golgi network (TGN) retrograde trafficking in both yeast and mammals. However, the exact mechanisms by which it regulates this transport route remain largely unknown. In this paper, we show that COG interacts directly with the target membrane SNARE (t-SNARE) Syntaxin 6 via the Cog6 subunit. In Cog6-depleted cells, the steady-state level of Syntaxin 6 was markedly reduced, and concomitantly, endosome-to-TGN retrograde traffic was significantly attenuated. Cog6 knockdown also affected the steady-state levels and/or subcellular distributions of Syntaxin 16, Vti1a, and VAMP4 and impaired the assembly of the Syntaxin 6-Syntaxin16-Vti1a-VAMP4 SNARE complex. Remarkably, overexpression of VAMP4, but not of Syntaxin 6, bypassed the requirement for COG and restored endosome-to-TGN trafficking in Cog6-depleted cells. These results suggest that COG directly interacts with specific t-SNAREs and positively regulates SNARE complex assembly, thereby affecting their associated trafficking steps.zh_CN
dc.language.isoen_USzh_CN
dc.publisherJ CELL BIOLzh_CN
dc.source.urihttp://dx.doi.org/10.1083/jcb.201102045zh_CN
dc.subjectMANNOSE 6-PHOSPHATE RECEPTORSzh_CN
dc.subjectOLIGOMERIC GOLGI-COMPLEXzh_CN
dc.subjectENDOPLASMIC-RETICULUMzh_CN
dc.subjectSNARE COMPLEXzh_CN
dc.subjectCONGENITAL DISORDERzh_CN
dc.subjectPROTEIN COMPLEXzh_CN
dc.subjectEARLY/RECYCLING ENDOSOMESzh_CN
dc.subjectSACCHAROMYCES-CEREVISIAEzh_CN
dc.subjectDEFICIENCY REVEALSzh_CN
dc.subjectTETHERING COMPLEXzh_CN
dc.titleThe COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transportzh_CN
dc.typeArticlezh_CN


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