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dc.contributor.authorHuang He-Qing
dc.contributor.author黄河清
dc.contributor.authorLu Yong-Jin
dc.contributor.authorLin Qing-Mei
dc.contributor.authorZhuo Hui-Qin
dc.contributor.authorHuang Hui-Ying
dc.date.accessioned2011-05-29T23:33:50Z
dc.date.available2011-05-29T23:33:50Z
dc.date.issued2007-09
dc.identifier.citationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(8):1105-1110zh_CN
dc.identifier.issn0253-3820
dc.identifier.urihttp://dx.doi.org/doi:10.1016/S1872-2040(07)60072-3
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/9263
dc.description.abstractEndopeptidease of Aplysia egg (AEE) was separated and purified by column chromatography using separation gel both DEAE-52 cellulose. The purity of AEE and its molecular weight of the subunit approximately 39. 0 kDa) were determined by Sephadex G-1 50 and SDS-polyacrylamid gel electrophoresis PAGE) methods. The results from MALDI-TOF mass spectrometry indicated that AEE(39) consisted of single subunit type ( M and its ratio of mass to charge ( m/z) was 12738. 17, 18108. 79 and 38221. 42, called [M3+], [M2+], and [M+] respectively. Using probe of insulin ( INS), a combined technology with both metal chelator EDTA and MALDI-TOF mass spectrometry was employed to measure molecular weight of AEE39 and to identify an endopeptidease with metal zinc, showing molecular weight with 38221. 42Da, called AEE,,. It was found that AEE 39 has the capacity for lysating INS, giving enzymolysis produces with m/z 1449. 51, 2085. 84, 4080. 41, 4165. 42. The software designed with ourselves was used to analyze the produces sequences and to identify the cleavage of INS. As a conclusion, It was found that the best sensitive cleavage for enzymolysis was Leu ( leucine) -X ( X: residues of amino acid), next one is Glu ( glutamic acid) -X. In addition, Phe(phenylalanine)-X, Asn(asparagine)-X and Ser(serine)-X in INS can be lysated once in a while. Compared to molecular structure both INS and attraction, it indicated that one of main functions of AEE39 was responsible for lysating the attraction in egg, which played an important role in informational intercourse, recalling recognizing, and mating. In addition, AEE(39) had another novel function for enzymolysis Leu-Leu in acidic peptide, which was a multifunctional ednopeptidease.zh_CN
dc.language.isoenzh_CN
dc.publisherSCIENCE CHINA PRESSzh_CN
dc.subjectAplysiazh_CN
dc.subjectendopeptideasezh_CN
dc.subjectmass spectrometryzh_CN
dc.subjectattractionzh_CN
dc.subjectinsulinzh_CN
dc.titleComposition, structure and function from endopeptidease of Aplysia egg analyzed with matrix assisted laser desorption ionization-time of flight-mass spectrometryzh_CN
dc.typeArticlezh_CN


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