ORMOSIL oxygen sensors on polystyrene microplate for dissolved oxygen measurement

Abstract

Oxygen sensors prepared from tetramethyl orthosilicate and dimethoxy dimethylsilane with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II) as the sensing dye were coated onto the well bottom surface of a 96-well polystyrene microtiter plate to give a high-throughput system for dissolved oxygen measurement. The oxygen sensors give linear Stern-Volmer calibration plots, and produce reliable and reproducible results in the determination Of IC50 values of drugs on a yeast model. (c) 2006 Elsevier B.V All rights reserved.