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dc.contributor.authorWei, W. L.zh_CN
dc.contributor.authorZheng, Z. H.zh_CN
dc.contributor.authorLiu, Y. Y.zh_CN
dc.contributor.authorZhu, X. S.zh_CN
dc.contributor.author郑忠辉zh_CN
dc.date.accessioned2015-07-22T07:13:34Z
dc.date.available2015-07-22T07:13:34Z
dc.date.issued1998zh_CN
dc.identifier.citationJournal of Fermentation and Bioengineering, 1998,86(4):395-399zh_CN
dc.identifier.otherISI:000077720800011zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/90445
dc.description.abstractThe response surface method (RSM) was used to optimize the medium for the production of inulinase by Kluyveromyces sp. Y-85. The inulinase production was adequately approximated with a full quadratic equation obtained from a four-factor-five-level central composite design. Analyses of the quadratic surfaces showed that in a 24-h fermentation at 30 degrees C, the maximum inulinase activity 59.5 U/ml appeared at extract of Jerusalem artichoke, urea, beef extract, corn steep liquor concentrations 8.0%, 2.0%, 0.2% and 4.0%, respectively. We further investigated the fermentation in 15 l fermenter and scaling-up in 1000 l tower fermenter. The inulinase activity in the scaling-up was 68.9 U/ml, which was the highest production reported at present, indicating that Kluyveromyces sp. Y-85 was an excellent strain for industrial production.zh_CN
dc.language.isoen_USzh_CN
dc.publisherSOC BIOSCIENCE BIOENGINEERING JAPANzh_CN
dc.source.urihttp://dx.doi.org/10.1016/S0922-338X(99)89011-4zh_CN
dc.subjectBETA-FRUCTOFURANOSIDASE INULASEzh_CN
dc.subjectASPERGILLUS-NIGERzh_CN
dc.subjectMARXIANUSzh_CN
dc.subjectBATCHzh_CN
dc.subjectOPTIMIZATIONzh_CN
dc.subjectFRAGILISzh_CN
dc.titleOptimizing the culture conditions for higher inulinase production by Kluyveromyces sp Y-85 and scaling-up fermentationzh_CN
dc.typeArticlezh_CN


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