Efficient enzymatic hydrolysis of the bagasse pulp prepared with active oxygen and MgO-based solid alkali
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The enzymatic hydrolysis of the bagasse pulp prepared from the treatment process with active oxygen and MgO-based solid alkali was studied. The hydrolysates were tested by IC (ionic chromatography) for the analysis of monosaccharide. Additionally, the changes of pulp before and after hydrolysis were characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), Kajaani cellulose automatic analyzer and atomic force microscopy (AFM) techniques. The results showed that an optimized sugar yield of 82.38% was obtained at the substrate concentration of 5% for 72 h with the enzyme dosage of 15 IU/g. Furthermore, as the length of the cellulose fiber decreased, the crystallinity of cellulose increased, and more depressions appeared on the surface of pulp after enzymatic hydrolysis. (C) 2013 Elsevier Ltd. All rights reserved.