Tris(2,2 '-bipyridyl)ruthenium(II) electrochemiluminescence sensor based on carbon nanotube/organically modified silicate films
- 化学化工－已发表论文 
In this paper, a novel electrochemiluminescence (ECL) sensor was constructed to determine herring sperm (HS) double-stranded (ds) DNA. Tetramethoxysilane and dimethyldimethoxysilane were selected as co-precursors to form an organically modified silicate (ORMOSIL) film for the immobilization of multimall carbon nanotubes (MWNTs) wrapped by poly(p-styrenesulfonate) (PSS), and then Tris(2,2'-bipyridyl)ruthenium(fl) (Ru(bpy)3 21) was successfully immobilized on a glassy carbon electrode via ion-association. PSS was employed to increase the conductivity of the ORMOSIL film and disperse the cut MWNTs, which were cut and shortened in a mixture of concentrated sulfuric and nitric acids, in the film. It was found that MWNTs could adsorb Ru(bPY)(3)(2+) and acted as conducting pathways to connect Ru(bpY)(3)(2+) sites to the electrode. MWNTs also played a key role as materials for the mechanical and thermal properties. The ECL performance of this modified electrode was evaluated in a flow injection analysis (FIA) system, and the detection limit (SIN= 3) for HS ds-DNA was 2.0 x 10(-7) g mL(-1) with a linear range from 1.34 x 10(-6) to 6.67 x 10(-4) g mL(-1) (R2 =0.9876). In addition, the ECL sensor presented excellent characteristics in terms of stability, reproducibility and application life. (C) 2007 Elsevier B.V. All rights reserved.