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dc.contributor.authorZhu, Zhizh_CN
dc.contributor.authorSong, Yanlingzh_CN
dc.contributor.authorLi, Congzh_CN
dc.contributor.authorZou, Yuanzh_CN
dc.contributor.authorZhu, Lingzh_CN
dc.contributor.authorAn, Yuanzh_CN
dc.contributor.authorYang, Chaoyong Jameszh_CN
dc.contributor.author朱志zh_CN
dc.contributor.author杨朝勇zh_CN
dc.date.accessioned2015-07-22T03:21:10Z
dc.date.available2015-07-22T03:21:10Z
dc.date.issued2014-06-17zh_CN
dc.identifier.citationANALYTICAL CHEMISTRY, 2014,86(12):5881-5888zh_CN
dc.identifier.otherWOS:000337643500043zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/89269
dc.descriptionNational Basic Research Program of China [2010CB732402, 2013CB933703]; National Science Foundation of China [91313302, 21205100, 21275122, 21075104]; National Instrumentation Program [2011YQ03012412]; Natural Science Foundation of Fujian Province, China [2013J01061]; Fundamental Research Funds for the Central Universities [2012121025]; National Science Foundation for Distinguished Young Scholars of China [21325522]zh_CN
dc.description.abstractA novel method, monoclonal surface display SELEX (MSD-SELEX), has been designed for simple, rapid, efficient, and cost-effective enrichment and identification of aptamers from a library of monoclonal DNA-displaying beads produced via highly parallel single-molecule emulsion PCR. The approach was successfully applied for the identification of high-affinity aptamers that bind specifically to different types of targets, including cancer biomarker protein EpCAM and small toxin molecule aflatoxin B-1. Compared to the conventional sequencing-chemical synthesis-screening work flow, MSD-SELEX avoids large-scale DNA sequencing, expensive and time-consuming DNA synthesis, and labor-intensive screening of large populations of candidates, thus offering a new approach for simple, rapid, efficient, and cost-effective aptamer identification for a wide variety of applications.zh_CN
dc.language.isoen_USzh_CN
dc.publisherAMER CHEMICAL SOCzh_CN
dc.source.urihttp://dx.doi.org/10.1021/ac501423gzh_CN
dc.subjectCELL ADHESION MOLECULEzh_CN
dc.subjectDNA APTAMERSzh_CN
dc.subjectBIOANALYTICAL APPLICATIONSzh_CN
dc.subjectCAPILLARY-ELECTROPHORESISzh_CN
dc.subjectCHEMICAL BIOLOGYzh_CN
dc.subjectSELECTIONzh_CN
dc.subjectCANCERzh_CN
dc.subjectLIGANDSzh_CN
dc.subjectAMPLIFICATIONzh_CN
dc.subjectEXPRESSIONzh_CN
dc.titleMonoclonal Surface Display SELEX for Simple, Rapid, Efficient, and Cost-Effective Aptamer Enrichment and Identificationzh_CN
dc.typeArticlezh_CN


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