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dc.contributor.authorLan, Y.zh_CN
dc.contributor.authorXu, X.zh_CN
dc.contributor.authorYang, F.zh_CN
dc.contributor.authorZhang, X.zh_CN
dc.contributor.author徐昕zh_CN
dc.contributor.author张弦zh_CN
dc.date.accessioned2015-07-22T03:19:59Z
dc.date.available2015-07-22T03:19:59Z
dc.date.issued2006-09zh_CN
dc.identifier.citationARCHIVES OF VIROLOGY, 2006,151(9):1723-1733zh_CN
dc.identifier.otherWOS:000239725000004zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/88546
dc.description.abstractIn an attempt to obtain the transcriptional profile of shrimp white spot syndrome virus (WSSV) genes, DNA microarray analysis was performed using amplified DNA fragments of the WSSV genome that covered most of the presumptive open reading frames (ORFs). Total RNAs were extracted from WSSV-infected crayfish (Cambarus clarkii) and reverse transcribed into cDNA and labeled with P-32-dATP. The DNA microarray detected 81.1% of the ORFs, and 47 of these were transcribed at 6 h post infection, suggesting they may be early genes. Some early genes were further confirmed by temporal analyses of gene transcription. Results in this study indicated the effectiveness of DNA microarray for discovering early viral genes.zh_CN
dc.language.isoen_USzh_CN
dc.publisherARCH VIROLzh_CN
dc.source.urihttp://dx.doi.org/10.1007/s00705-006-0751-xzh_CN
dc.subjectPOLYMERASE-CHAIN-REACTIONzh_CN
dc.subjectIN-SITU HYBRIDIZATIONzh_CN
dc.subjectBACULOVIRUS WSBVzh_CN
dc.subjectGENOME SEQUENCEzh_CN
dc.subjectIDENTIFICATIONzh_CN
dc.subjectPRAWNzh_CN
dc.titleTranscriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarrayzh_CN
dc.typeArticlezh_CN


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