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dc.contributor.authorSun, Pingzh_CN
dc.contributor.authorClamp, John C.zh_CN
dc.contributor.authorXu, Dapengzh_CN
dc.contributor.authorHuang, Bangqinzh_CN
dc.contributor.authorShin, Mann Kyoonzh_CN
dc.contributor.authorTurner, Franziskazh_CN
dc.contributor.author黄邦钦zh_CN
dc.date.accessioned2015-07-22T03:12:14Z
dc.date.available2015-07-22T03:12:14Z
dc.date.issued2013-Nov 22zh_CN
dc.identifier.citationPROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2013,280(1771)zh_CN
dc.identifier.otherWOS:000330324900002zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/87927
dc.descriptionNatural Science Foundation of China [31372167, 41306125]; National Science Foundation [DEB-0716348]; Basic Science Research Program through NRF; Government of Korea (MEST) [2012R1A1A2005751]zh_CN
dc.description.abstractVorticella includes more than 100 currently recognized species and represents one of the most taxonomically challenging genera of ciliates. Molecular phylogenetic analysis of Vorticella has been performed so far with only sequences coding for small subunit ribosomal RNA (SSU rRNA); only a few of its species have been investigated using other genetic markers owing to a lack of similar sequences for comparison. Consequently, phylogenetic relationships within the genus remain unclear, and molecular discrimination between morphospecies is often difficult because most regions of the SSU rRNA gene are too highly conserved to be helpful. In this paper, we move molecular systematics for this group of ciliates to the infrageneric level by sequencing additional molecular markers-fast-evolving internal transcribed spacer (ITS) regions-in a broad sample of 66 individual samples of 28 morphospecies of Vorticella collected from Asia, North America and Europe. Our phylogenies all featured two strongly supported, highly divergent, paraphyletic clades (I, II) comprising the morphologically defined genus Vorticella. Three major lineages made up clade I, with a relatively well-resolved branching order in each one. The marked divergence of clade II from clade I confirms that the former should be recognized as a separate taxonomic unit as indicated by SSU rRNA phylogenies. We made the first attempt to elucidate relationships between species in clade II using both morphological and multi-gene approaches, and our data supported a close relationship between some morphospecies of Vorticella and Opisthonecta, indicating that relationships between species in the clade are far more complex than would be expected from their morphology. Different patterns of helix III of ITS2 secondary structure were clearly specific to clades and subclades of Vorticella and, therefore, may prove useful for resolving phylogenetic relationships in other groups of ciliates.zh_CN
dc.language.isoen_USzh_CN
dc.publisherROYAL SOCzh_CN
dc.source.urihttp://dx.doi.org/10.1098/rspb.2013.1177zh_CN
dc.subjectSUBUNIT RIBOSOMAL-RNAzh_CN
dc.subjectSECONDARY STRUCTUREzh_CN
dc.subjectCILIOPHORAzh_CN
dc.subjectEVOLUTIONzh_CN
dc.subjectPERITRICHIAzh_CN
dc.subjectSEQUENCEzh_CN
dc.subjectOLIGOHYMENOPHOREAzh_CN
dc.subjectRECONSTRUCTIONzh_CN
dc.subjectPREDICTIONzh_CN
dc.subjectPROTOZOAzh_CN
dc.titleAn ITS-based phylogenetic framework for the genus Vorticella: finding the molecular and morphological gaps in a taxonomically difficult groupzh_CN
dc.typeArticlezh_CN


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