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dc.contributor.authorLi, Weizh_CN
dc.contributor.authorGao, Kunshanzh_CN
dc.contributor.authorBeardall, Johnzh_CN
dc.contributor.author高坤山zh_CN
dc.date.accessioned2015-07-22T03:12:07Z
dc.date.available2015-07-22T03:12:07Z
dc.date.issued2012-12-07zh_CN
dc.identifier.citationPLOS ONE, 2012,7(12)zh_CN
dc.identifier.otherWOS:000312064100133zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/87849
dc.descriptionNational Basic Research Program of China [2009CB421207, 2011CB200902]; National Natural Science Foundation [40930846, 41120164007]; Program for Changjiang Scholars and Innovative Research Team [IRT0941]; China-Japan collaboration project from MOST [S2012GR0290]zh_CN
dc.description.abstractClimate change is expected to bring about alterations in the marine physical and chemical environment that will induce changes in the concentration of dissolved CO2 and in nutrient availability. These in turn are expected to affect the physiological performance of phytoplankton. In order to learn how phytoplankton respond to the predicted scenario of increased CO2 and decreased nitrogen in the surface mixed layer, we investigated the diatom Phaeodactylum tricornutum as a model organism. The cells were cultured in both low CO2 (390 mu atm) and high CO2 (1000 mu atm) conditions at limiting (10 mu mol L-1) or enriched (110 mu mol L-1) nitrate concentrations. Our study shows that nitrogen limitation resulted in significant decreases in cell size, pigmentation, growth rate and effective quantum yield of Phaeodactylum tricornutum, but these parameters were not affected by enhanced dissolved CO2 and lowered pH. However, increased CO2 concentration induced higher rETR(max) and higher dark respiration rates and decreased the CO2 or dissolved inorganic carbon (DIC) affinity for electron transfer (shown by higher values for K-1/2 DIC or K-1/2 CO2). Furthermore, the elemental stoichiometry (carbon to nitrogen ratio) was raised under high CO2 conditions in both nitrogen limited and nitrogen replete conditions, with the ratio in the high CO2 and low nitrate grown cells being higher by 45% compared to that in the low CO2 and nitrate replete grown ones. Our results suggest that while nitrogen limitation had a greater effect than ocean acidification, the combined effects of both factors could act synergistically to affect marine diatoms and related biogeochemical cycles in future oceans.zh_CN
dc.language.isoen_USzh_CN
dc.publisherPLOS ONEzh_CN
dc.source.urihttp://dx.doi.org/10.1371/journal.pone.0051590zh_CN
dc.subjectCARBON CONCENTRATING MECHANISMSzh_CN
dc.subjectMARINE-PHYTOPLANKTONzh_CN
dc.subjectULTRAVIOLET-RADIATIONzh_CN
dc.subjectCO2 CONCENTRATIONzh_CN
dc.subjectPHOTOSYNTHESISzh_CN
dc.subjectSEAWATERzh_CN
dc.subjectGROWTHzh_CN
dc.subjectIRONzh_CN
dc.subjectPHzh_CN
dc.subjectAVAILABILITYzh_CN
dc.titleInteractive Effects of Ocean Acidification and Nitrogen-Limitation on the Diatom Phaeodactylum tricornutumzh_CN
dc.typeArticlezh_CN


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