Multilayer chitosan-based open tubular capillary anion exchange column with integrated monolithic capillary suppressor
Foss, Frank W., Jr.
Dasgupta, Purnendu K.
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We describe a multilayered open tubular anion exchange column fabricated by alternately pumping solutions of chitosan and glutaraldehyde. The column is terminated in an integrally bonded monolithic suppressor cast around a mandrel of a tungsten wire, composed of an acrylic acid (AA)-ethylene dimethacrylate (EDMA) monolith that is made with sufficient porogen for the monolith to function as a membrane. For a 4.5 m long 75 mu m bore column coated with 24 successive layers of the condensation polymer (estimated to contain similar to 72 molecular layers) and coupled to 1 cm length of a suppressor fabricated with 55-60% AA, effective separation of several common anions (F-, Cl-, NO2-, Br-, NO3-, average number of theoretical plates similar to 12,000) and adequate suppression of 1 mM KOH used as eluent was observed at a flow rate of 800 nL min(-1) to obtain sub-picomol detection limits at an operating pressure of similar to 1 bar. The separation is not time efficient but the system can be meritorious in unique niche applications where a small form factor is desired and liquid volume and power consumption are more important than separation speed. (C) 2011 Elsevier B.V. All rights reserved.