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dc.contributor.authorChen, Weiyuzh_CN
dc.contributor.authorZhang, Hengzh_CN
dc.contributor.authorGu, Lizh_CN
dc.contributor.authorLi, Fangzh_CN
dc.contributor.authorYang, Fengzh_CN
dc.contributor.author陈维宇zh_CN
dc.date.accessioned2015-07-22T03:11:52Z
dc.date.available2015-07-22T03:11:52Z
dc.date.issued2012-11-08zh_CN
dc.identifier.citationDISEASES OF AQUATIC ORGANISMS, 2012,101(2):167-171zh_CN
dc.identifier.otherWOS:000310843100010zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/87729
dc.descriptionNational Basic Research Program of China (973 Program) [2012CB114401]; Natural Science Foundation of China [31072243]; China Agriculture Research System-47zh_CN
dc.description.abstractThe structural stability of white spot syndrome virus (WSSV) capsids at high salinity, high temperature and various pH values was studied. To obtain the viral capsids, the nucleocapsids were treated with high salinity. The results showed that high salinity treatment can cause the dissociation of VP15 and most of VP95 from the nucleocapsid, but there were no noticeable alterations in morphology and ultrastructure of the nucleocapsid and capsid. The capsids retained morphological integrity at temperatures <45 degrees C but became aberrant at >60 degrees C. In addition, the capsids were relatively resistant to strong acid conditions and were tolerant to a broad pH range of 1 to 10. However, morphological change occurred at pH 10.5. The capsids broke up into small pieces at pH 11 and completely degraded in 0.1 and 1.0 M NaOH. These results indicated that the WSSV capsid is acid-stable and alkali-labile.zh_CN
dc.language.isoen_USzh_CN
dc.publisherDIS AQUAT ORGANzh_CN
dc.source.urihttp://dx.doi.org/10.3354/dao02511zh_CN
dc.subjectPROTEOMIC ANALYSISzh_CN
dc.subjectGENOME SEQUENCEzh_CN
dc.subjectPROTEIN VP15zh_CN
dc.subjectSHRIMPzh_CN
dc.subjectENVELOPEzh_CN
dc.subjectGENEzh_CN
dc.subjectIDENTIFICATIONzh_CN
dc.subjectBINDINGzh_CN
dc.subjectNUCLEIzh_CN
dc.subjectVIRIONzh_CN
dc.titleEffects of high salinity, high temperature and pH on capsid structure of white spot syndrome viruszh_CN
dc.typeArticlezh_CN


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