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dc.contributor.author张兵zh_CN
dc.contributor.author夏春zh_CN
dc.contributor.author陈福zh_CN
dc.date.accessioned2011-04-26T08:24:10Z
dc.date.available2011-04-26T08:24:10Z
dc.date.issued2008-03zh_CN
dc.identifier.citation中国微创外科杂志 Chinese Journal of Minimally Invasive Surgery,2008,8(3):273-275zh_CN
dc.identifier.issn1009-6604zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8521
dc.description.abstract目的研究大鼠腓肠肌内蛋白激酶B(Akt/PKB)各亚型伴随年龄变化的表达差异。方法用反转录PCR(RT-PCR)、免疫印迹(westernblotting)检测老年组(30月龄)和青年组(6月龄)大鼠腓肠肌内Akt各亚型mRNA和蛋白表达,并统计分析比较。结果(1)30月龄组大鼠腓肠肌内Akt1与Akt2的mRNA水平均高于6月龄组(t=7.124,P=0.000;t=2.598,P=0.021),Akt3差异无显著性(t=0.460,P=0.653)。(2)两组间Akt1蛋白表达水平无显著变化(t=0.469,P=0.646),30月龄组Akt1的Thr308磷酸化水平低于6月龄组的表达,有显著差异(t=-9.861,P=0.000);30月龄组Akt2的蛋白水平高于6月龄组的表达,有显著差异(t=7.522,P=0.000);Akt3蛋白表达在两组间无差异(t=0.058,P=0.955)。结论大鼠腓肠肌中Akt三种亚型伴随年龄改变表达存在差异,推测Akt各亚型在此过程中可能发挥不同功效。 【英文摘要】 Objective To study the expression of protein kinase B (Akt/PKB) isoforms in the gastrocnemius muscle of rats at different ages. Methods The expression levels of mRNA and protein of three Akt isoforms in the gastrocnemius muscle of 30-month-old and 6-month-old rats were detected respectively by RT-PCR and Western blotting. Data were analyzed statistically. Results (1) The levels of Akt1 and Akt2 mRNA in the 30-month-old rats was significantly higher than those in the 6-month-old rats (t=7.124, P=0.000; t=2.5...zh_CN
dc.description.sponsorship福建省自然科学基金计划资助项目(C0310001)zh_CN
dc.language.isozhzh_CN
dc.publisher北京大学zh_CN
dc.subjectAkt1zh_CN
dc.subjectAkt2zh_CN
dc.subjectAkt3zh_CN
dc.subject大鼠zh_CN
dc.subject腓肠肌zh_CN
dc.subject萎缩zh_CN
dc.subjectRatszh_CN
dc.subjectGastrocnemius musclezh_CN
dc.subjectAtrophyzh_CN
dc.title大鼠腓肠肌内蛋白激酶B各亚型表达的年龄差异zh_CN
dc.title.alternativeExpression of Protein Kinase B Isoforms in the Gastrocnemius Muscle of Rats at Different Ageszh_CN
dc.typeArticlezh_CN


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