Show simple item record

dc.contributor.author陈锦萍zh_CN
dc.contributor.author王效民zh_CN
dc.contributor.author张忠英zh_CN
dc.contributor.author游攀zh_CN
dc.contributor.author李涌zh_CN
dc.contributor.author黄建炜zh_CN
dc.contributor.author吴绍峰zh_CN
dc.contributor.author傅锦波zh_CN
dc.date.accessioned2011-04-26T08:24:10Z
dc.date.available2011-04-26T08:24:10Z
dc.date.issued2007zh_CN
dc.identifier.citation临床检验杂志 Chinese Journal of Clinical Laboratory Science,2007,25(5):364-366zh_CN
dc.identifier.issn1001-764Xzh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8517
dc.description.abstract目的检测染色体普通型脆性位点FRA16D(16q23.3-q24.1)上WWOX(WW domain containing oxidoreductase)基因在原发性肝细胞癌(HCC)中的表达及意义。方法用RT-PCR、荧光定量PCR方法分别对40例原发性肝细胞癌癌组织(以下简称肝癌组织)、癌旁组织中WWOX基因的Δ6-8变异情况及其mRNA表达差异进行检测。结果WWOX mRNA在肝癌组织的表达(0.318±0.0559)显著低于癌旁组织(1.0),P<0.05;而且在肝癌组织中Δ6-8变异体的出现频率为20%(8/40),显著高于癌旁组织2.5%(1/40),P<0.05。结论WWOX基因的低表达、变异可能在肝癌的发生发展中起重要作用,并有可能作为肝癌的早期诊断指标之一。 【英文摘要】 Objective To evaluate the expression and significance of tumor suppressor gene(WWOX)in hepatocellular carcinoma(HCC)tissues.Methods Variant delta 6-8 of WWOX in patients with hepatocellular carcinoma was detected by RT-PCR and the expression of WWOX mRNA was examined by fluorescent quantitative PCR(FQ-PCR).Results The expression of WWOX mRNA in HCC patients(0.318±0.0559)was significantly lower than that in para-carcinoma tissue(P<0.05).Variant delta 6-8 was found in HCC and its frequency(20%,8/40)was sig...zh_CN
dc.language.isozhzh_CN
dc.publisher江苏省医学会zh_CN
dc.subject肝细胞癌zh_CN
dc.subjectWWOX基因zh_CN
dc.subject抑癌基因zh_CN
dc.subject脆性位点zh_CN
dc.subjecthepatocellular carcinomazh_CN
dc.subjectwwox genezh_CN
dc.subject; tumor suppressor genezh_CN
dc.subjectfrigile siteszh_CN
dc.titlePCR检测原发性肝细胞癌WWOX基因表达及意义zh_CN
dc.title.alternativeStudy on expression and significance of tumor suppressor gene WWOX in hepatocellular carcinoma tissues by real-time fluorescent quantitative PCRzh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record