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dc.contributor.author宋玉国zh_CN
dc.contributor.author李文珠zh_CN
dc.contributor.author李迎zh_CN
dc.contributor.author陈彩霞zh_CN
dc.contributor.author庄国洪zh_CN
dc.date.accessioned2011-04-26T08:24:10Z
dc.date.available2011-04-26T08:24:10Z
dc.date.issued2007zh_CN
dc.identifier.citation中国免疫学杂志 Chinese Journal of Immunology,2007,23(1):38-41zh_CN
dc.identifier.issn1000-484Xzh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8514
dc.description.abstract目的探讨FasL、Anti-DR5mAb对肿瘤细胞Hela、BGC823、MCF-7、L342、H9101、D6的杀伤作用及机制。方法采用RT-PCR、MTT比色法、电泳、DNA倍体分析、Westernblot等方法。结果H9101、Hela细胞株DR5mRNA水平有表达,D6细胞无表达;H9101、L342细胞株FasmRNA水平有表达,D6细胞无表达。H9101、L342细胞株对FasL、Anti-DR5mAb敏感并呈剂量依赖性;MCF-7、BGC823细胞株对FasL敏感,对Anti-DR5mAb相对敏感。Hela对FasL相对敏感,对Anti-DR5mAb敏感;D6对两种凋亡诱导剂耐受。结论FasL、Anti-DR5mAb能不同程度地诱导肿瘤细胞凋亡,其机制与Fas、DR5、Caspase-8、Bcl-2的表达有关。 【英文摘要】 Objective:To study the cytotoxic effects on tumor cell lines Hela,BGC823,MCF-7,L342,H9101,D6 induced by Fas ligand and anti-human DR5 monoclonal antibodies(Anti-DR5 mAb) and the underlying mechanism.Methods:Fas/DR5 mRNA were detected by RT-PCR. Cytotoxicity exerted by FasL/Anti-DR5 mAb on tumor cell lines was measured by MTT colorimetry and the induced apoptosis was determined by agarose gel electrophoresis.Results:The expression of DR5 on BGC823 and Hela cells were higher and DR5 didn’t express in D6. The ...zh_CN
dc.description.sponsorship厦门大学科研启动基金(No.Z03103)资助zh_CN
dc.language.isozhzh_CN
dc.publisher中国免疫学会;吉林省卫生厅zh_CN
dc.subjectFasLzh_CN
dc.subject肿瘤坏死因子相关凋亡诱导配体zh_CN
dc.subject死亡受体zh_CN
dc.subject凋亡zh_CN
dc.subjectTRAILzh_CN
dc.subjectDeath receptorzh_CN
dc.subjectApoptosiszh_CN
dc.titleFasL、抗人DR5单克隆抗体诱导肿瘤细胞凋亡的研究zh_CN
dc.title.alternativeFas ligand and Anti-human DR5 monoclonal antibody induce tumor cell lines apoptosiszh_CN
dc.typeArticlezh_CN


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