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dc.contributor.author王芳zh_CN
dc.contributor.author齐建国zh_CN
dc.contributor.author王廷华zh_CN
dc.contributor.author章为zh_CN
dc.contributor.author孙玲zh_CN
dc.date.accessioned2011-04-26T08:24:09Z
dc.date.available2011-04-26T08:24:09Z
dc.date.issued2008-05zh_CN
dc.identifier.citation神经解剖学杂志 Chinese Journal of Neuroanatomy,2008,2493):271-276zh_CN
dc.identifier.issn1000-7547zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8509
dc.description.abstract为研究损伤脊髓神经细胞凋亡和相联系的caspase-3的表达,用原位末端标记法(TUNEL)检测了成年大鼠胸脊髓(T9)半横断损伤(hSCI)后脊髓神经细胞的凋亡变化,并用免疫组织化学染色方法观察了caspase-3的表达。结果显示:hSCI后6h损伤侧脊髓腹角即出现较多的TUNEL阳性神经细胞,并持续至5周。损伤脊髓的吻侧段于手术后4d达高峰,尾侧段脊髓于伤后1d达高峰。与假手术组相比,在所有观察的时间点TUNEL阳性神经细胞的数量在损伤侧均有明显增加。caspase-3免疫组化染色显示,与假手术组相比,伤后6h损伤侧脊髓腹角caspase-3表达也明显增加,并持续至5周。以上结果提示hSCI后,脊髓可出现神经细胞凋亡,且此凋亡的发生可能与caspase-3的表达有关。 【英文摘要】 To study neuronal apoptosis and the associated caspase-3 expression in the injured spinal cord,the thoracic cord(T9) underwent hemisected(hSCI) operation in adult rats.Neuronal apoptosis and caspase-3 expression were investigated by using terminal deoxyn-ucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) method and immunohistochemical technique.The results showed that the expression of TUNEL positive neurons,which were found in ventral horn of the spinal cord on the lesion side beginning from the...zh_CN
dc.language.isozhzh_CN
dc.publisher中国解剖学会zh_CN
dc.subject细胞凋亡zh_CN
dc.subjectcaspase-3zh_CN
dc.subject免疫组织化学zh_CN
dc.subjectTUNEL染色zh_CN
dc.subject半横断损伤zh_CN
dc.subject脊髓zh_CN
dc.subject大鼠zh_CN
dc.subjectapoptosiszh_CN
dc.subjectimmunohistochemistryzh_CN
dc.subjectTUNEL stainingzh_CN
dc.subjectspinal cordzh_CN
dc.subjectratzh_CN
dc.subjecthemitransection injuryzh_CN
dc.title大鼠脊髓半横断损伤后脊髓神经细胞凋亡与caspase-3的表达zh_CN
dc.title.alternativeNeuronal apoptosis and caspase-3 expression in the spinal cord after hemitransection injury in ratszh_CN
dc.typeArticlezh_CN


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