高纯河豚毒素对蛙坐骨神经动作电位的影响
Effects of high-purity tetrodotoxin on compound action potential of sciatic nerve isolated from frog
Abstract
用电生理学方法观察了4种不同浓度的高纯河豚毒素(tetrodotoxin,TTX)对蛙离体坐骨神经动作电位的影响.实验结果表明:1μmol/dm3TTX在给药后7 min能显著抑制动作电位的上相幅度(P<0.05),但在给药后10 min才显著抑制动作电位的下相幅度(P<0.01);20μmol/dm3TTX在给药后3 min内能完全抑制动作电位的形成.1μmol/dm3TTX在给药后1~5min对动作电位传导速度的影响没有统计学意义,5μmol/dm3TTX在5min时可显著抑制传导速度(P<0.05),而10μmol/dm3TTX在2min时便显著抑制传导速度(P<0.05).实验结果提示高纯河豚毒素对蛙离体坐骨神经动作电位的影响存在剂量效应关系.
【英文摘要】 Using electro-physiological method,the effects of high-purity tetrodotoxin(TTX) in four different concentrations on action potential of sciatic nerve isolated from frog in vitro were observed.The results showed that 1μmol/dm3 TTX could significantly inhibit the amplitude of first phase of action potential in 7min(P<0.05) after giving medicine and the amplitude of second phase in 10min(P<0.01);20μmol/dm3 TTX could completely inhibit action potential in 3min.1μmol/dm3 TTX showed no difference on conduct...