• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 医学院
  • 医学院-已发表论文
  • View Item
  •   DSpace Home
  • 医学院
  • 医学院-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

RGD-tTF水基磁流体的制备及其活性研究
Studies of preparation and properties of RGD-tTF water-based ferrofluids

Thumbnail
Full Text
RGD_tTF水基磁流体的制备及其活性研究.pdf (304.4Kb)
Date
2008
Author
颜江华
黄志平
王生育
王勇军
李文珠
Collections
  • 医学院-已发表论文 [2663]
Show full item record
Abstract
目的探讨RGD-tTF水基磁流体通过磁场和RGD多肽在体外对内皮细胞双靶向的功能。方法通过化学沉淀法以柠檬酸钠为表面活性剂制备水基磁流体(MnFe2O4),弱酸改性后与重组的RGD-tTF融合蛋白结合,利用H-600透射电镜观测纳米粒径,以SUQID鉴定磁性,用MTT法、因子X活化检测和流式细胞仪检测RGD-tTF磁流体生物活性。结果成功制备出的水基磁流体能在磷酸盐缓冲液中稳定分布且具有生物兼容性,实验表明RGD-tTF与水基磁流体结合后对RGD和tTF生物活性均无显著影响,并证实在磁场的作用下能实现了水基磁流体对RGD-tTF的定位作用。结论一种具有内皮细胞双靶向功能的RGD-tTF水基磁流体已制备成功。 【英文摘要】 Purpose To study the property of RGD-tTF water-based ferrofluids double targeting EC304 cells in vitro by magnet and RGD peptide.Methods Water-based ferrofluids(MnFe2O4) were prepared by chemical precipitation method using citrate as surfactant, dispersed in weak acid to create surface charges,and coated with recombinant RGD-tTF protein.Proteins coated ferrofluids were characterized by H-600 transmission electron microscopy(TEM)and Superconducting Quantum Interference Device(SQUID),and its biological activi...
Citation
中国生化药物杂志 Chinese Journal of Biochemical Pharmaceutics,2008,29(4):229-232
URI
https://dspace.xmu.edu.cn/handle/2288/8489

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies