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dc.contributor.author庄国洪zh_CN
dc.contributor.author宋玉国zh_CN
dc.contributor.author毕胜利zh_CN
dc.contributor.author杜柏榕zh_CN
dc.contributor.author朱迅zh_CN
dc.date.accessioned2011-04-26T08:24:06Z
dc.date.available2011-04-26T08:24:06Z
dc.date.issued2007zh_CN
dc.identifier.citation细胞与分子免疫学杂志 Chinese Journal of Cellular and Molecular Immunology,2007,23(2):120-123zh_CN
dc.identifier.issn1007-8738zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8482
dc.description.abstract目的探讨亚毒性剂量的阿霉素影响抗DR4、DR5单克隆抗体(mAb)FMU1.4和FMU1.5诱导3株神经胶质瘤细胞株U343(TRAIL敏感株)、U138(TRAIL部分敏感株)及U373(TRAIL耐受株)凋亡的作用及可能的机制。方法采用流式细胞术检测DR4、DR5的表达及神经胶质瘤细胞中DNA倍增。用MTT比色法检测mAbFMU1.4和FMU1.5对3株神经胶质瘤细胞增殖的抑制作用。用共聚焦显微镜观察3株细胞中Ca2+的浓度。以Westernblot检测细胞内色素C、FLIP[FLICE-inhibitoryprotein,为一组含有死亡效应结构域(DED)的胞浆蛋白]的表达。结果亚毒性剂量的阿霉素作用后,DR5、DR4在3株细胞中的表达提高;而mAbFMU1.4、FMU1.5诱导U138和U373细胞凋亡的作用增强,细胞内细胞色素C的表达提高,FLIP的表达降低,Ca2+浓度增加。结论亚毒性剂量的阿霉素与抗DR4、DR5mAb联合应用后,可提高mAb诱导靶细胞凋亡的效应,其作用机制与DR4、DR5、细胞色素C、FLIP的表达及Ca2+的含量有关。 【英文摘要】 AIM: To study the cytotoxic effects of doxorubicin on apoptosis in glioma cell lines U343, U138, U373 induced by anti-human DR4/DR5 monoclonal antibodies (FMU1.4/FMU1.5) and the underlying mechanism. METHODS: Expression of DR4/DR5 was quantitated by flow cytometry. Cytotoxicity exerted by FMU1.4/FMU1.5 on three cell lines was measured by MTT colorimetry and the induced apoptosis was determined by agarose gel electrophoresis. The expression of cytochrome C, FLIP and Ca 2+ concentration were also measured. RE...zh_CN
dc.description.sponsorship教育部跨世纪优秀人才培养计划资助项目(2000年)zh_CN
dc.language.isozhzh_CN
dc.publisher中国免疫学会;第四军医大学zh_CN
dc.subjectTNF相关凋亡诱导配体zh_CN
dc.subject死亡受体zh_CN
dc.subject凋亡zh_CN
dc.subject阿霉素zh_CN
dc.subjectTRAILzh_CN
dc.subjectdeath receptorzh_CN
dc.subjectapoptosiszh_CN
dc.subjectdoxorubicinzh_CN
dc.title亚毒性剂量阿霉素提高抗人DR4、DR5抗体诱导的神经胶质瘤细胞凋亡的研究zh_CN
dc.title.alternativeEnhancement of glioma cell apoptosis induced by anti-human DR5/DR4 monoclonal antibodies by sub-toxic dose of doxorubicin in humanzh_CN
dc.typeArticlezh_CN


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