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dc.contributor.author付萍zh_CN
dc.contributor.author李晴zh_CN
dc.contributor.author杨占秋zh_CN
dc.contributor.author陈文zh_CN
dc.contributor.author肖红zh_CN
dc.date.accessioned2011-04-26T08:24:05Z
dc.date.available2011-04-26T08:24:05Z
dc.date.issued2008-07zh_CN
dc.identifier.citation武汉大学学报(医学版) Medical Journal of Wuhan University.2008,29(4):423-531zh_CN
dc.identifier.issn1671-8852zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8481
dc.description.abstract目的:提高汉坦病毒(HV)的检出率。方法:设计了两对新的汉坦病毒(HV)通用引物,建立新的RT-PCR方法,对流行于湖北省不同地区的肾综合征出血热(HFRS)患者血清进行了检测。结果:在对166份血清中HV RNA的检测显示HTN型阳性率为80.1%(133/166),SEO型为19.9%(33/166);对不同浓度的HV RNA检测结果显示,本法最低可检测出血清中72 pg病毒基因组。结论:说明本法用于检测HFRS患者血清中HV RNA的特异性和敏感性均很高,且我国中部地区HFRS流行以HTN为优势型,这一结果不仅为HFRS的诊断提供了新的手段,也为HFRS的防治提供了新的依据。 【英文摘要】 Objective: To improve the detection rate of Hantavirus.Methods: Two new pairs of Hantavirus universal primers was designed to detect the Hantavirus genome RNA in hemorrhagic fever with renal syndrome(HFRS) patient s serum in Hubei Province by RT-PCR.Results: In the 166 serum,80.1%(133/166) were detected to be HTN and 19.9%(33/166) were SEO in serology typing.At the same time,even a low level of Hantavirus genome RNA(≥72 pg) could be detected by this method.Conclusion: It is an excellent method to detect Han...zh_CN
dc.description.sponsorship国家“863”计划(编号:2007AA02Z465);; 国家自然科学基金资助项目(编号:30770096)zh_CN
dc.language.isozhzh_CN
dc.publisher武汉大学zh_CN
dc.subject汉坦病毒zh_CN
dc.subjectPCRzh_CN
dc.subject引物设计zh_CN
dc.subject肾综合征出血热zh_CN
dc.subjectHantaviruszh_CN
dc.subjectPrimers Designzh_CN
dc.subjectHemorrhagic Fever with Renal Syndromezh_CN
dc.title用新型汉坦病毒通用引物检测HFRS患者血清中病毒核酸zh_CN
dc.title.alternativeDetection of Hantavirus Genome by New Pairs of Hantavirus Universal Primers in HFRS Patients Serumzh_CN
dc.typeArticlezh_CN


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