姜黄素对Ⅱ相酶GST及NQO酶活性的诱导及其机制(英文)

Abstract

研究姜黄素对Ⅱ相酶谷胱甘肽转移酶(GST)及NADP(H)醌氧化还原酶(NQO)活性的影响及其诱导机制。用光谱法检测细胞GST酶和NQO酶的活性,以及还原型谷胱甘肽(GSH)的含量;利用蛋白印迹法检测核转录因子Nrf2在胞浆与胞核的分布;采用凝胶电泳迁移率分析法(EMSA)检测Nrf2与Ⅱ相酶基因抗氧化反应序列(ARE)结合活性。不同浓度的姜黄素(10～30μmol.L-1)刺激结肠腺癌HT-29细胞后,能显著诱导GST酶及NQO酶活性的增加,同时能迅速提高细胞内GSH的含量;蛋白印迹和凝胶电泳迁移率结果显示,姜黄素诱导细胞核内转录因子Nrf2积聚,Nrf2-ARE的结合活性增加。姜黄素诱导的Ⅱ相酶GST酶及NQO酶活性增加与促进转录因子Nrf2由胞浆向胞核发生转位分布和增强Nrf2-ARE结合活性有关。 【英文摘要】 This study is to investigate the effect of curcumin on the induction of glutathione S-transferases(GST) and NADP(H):quinone oxidoreductase(NQO) and explore their possible molecular mechanism.The activity of GST,NQO and cellular reduced glutathione(GSH) content were measured by spectrophotometrical methods.Cellular changes in the distribution of NF-E2 related factor 2(Nrf2) were detected by Western blotting analysis.Nrf2-AREs (antioxidant-responsive elements) binding activity was examined by electrophoretic ...