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dc.contributor.author谢莲英zh_CN
dc.contributor.author张长弓zh_CN
dc.contributor.author吴娜zh_CN
dc.contributor.author王臻zh_CN
dc.contributor.author颜江华zh_CN
dc.date.accessioned2011-04-26T08:24:04Z
dc.date.available2011-04-26T08:24:04Z
dc.date.issued2007-06zh_CN
dc.identifier.citation厦门大学学报(自然科学版)Journal of Xiamen University(Natural Science),2007,46(1):96-99zh_CN
dc.identifier.issn0438-0479zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/8473
dc.description.abstract根据脂联素cDNA序列设计并合成9条寡聚核苷酸片段,经重叠PCR技术获得重组人脂联素基因.构建pET-22b(+)/ADPN表达载体,转化大肠杆菌BL21(DE3),IPTG诱导表达.目的蛋白以包涵体的形式表达,表达量占菌体总蛋白的30%以上.表达产物通过Ni-NTA柱纯化,SDS-PAGE分析显示,其分子量约为30 ku,与预期结果一致.内皮细胞生长抑制实验证实,重组人脂联素生理浓度下具有抑制人内皮细胞生长的活性并呈时间依赖性.以上工作为进一步研究脂联素的生物学功能奠定了基础. 【英文摘要】 According to the cDNA sequence encoding the human Adiponectin(ADPN),nine oligonucleotides were designed and synthesized.The ADPN gene was obtained by overlapping PCR technique.The expression vector pET22b(+)/ADPN was constructed and transformed into E.coli BL21(DE3).The ADPN protein was expressed as inclusion bodies with the yield of more than 30% of total bacterial proteins under IPTG induction.The expression products were purified by Ni-NTA affinity column,SDS-PAGE analysis showed that the relative molecu...zh_CN
dc.description.sponsorship教育部出国留学人员启动资金资助zh_CN
dc.language.isozhzh_CN
dc.publisher厦门大学zh_CN
dc.subject脂联素zh_CN
dc.subject基因构建zh_CN
dc.subject蛋白表达zh_CN
dc.subject生物活性;zh_CN
dc.subjectADPNzh_CN
dc.subjectgene constructionzh_CN
dc.subjectprotein expressionzh_CN
dc.subjectbiology activityzh_CN
dc.title重组人脂联素基因构建、表达、纯化及活性鉴定zh_CN
dc.title.alternativeGene Construction,Expression and Activity Analysis of Recombinant Human Adiponectinzh_CN
dc.typeArticlezh_CN


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