5氮胞苷与丁酸抑制人肝癌细胞转移的实验研究

Abstract

目的 了解 5氮胞苷和丁酸对肝癌细胞转移及肝癌细胞蛋白表达的影响。方法 使用 5氮胞苷与丁酸处理人肝癌细胞株HepG2及Hep3B ,观察处理后肝癌细胞转移、粘附及增殖的改变以及细胞周期抑制蛋白p2 1waf1、p2 7kip1和p5 3表达的改变。结果 5氮胞苷和丁酸联合使用能够完全阻断两种肝癌细胞的转移 ,转移细胞数均为 0 /膜 ;而对照组HepG2和Hep3B转移细胞数分别为(91± 17) /膜和 (117± 74) /膜 ,P <0 0 1;两种药物均可抑制肝癌细胞的粘附及增殖 ;接种后 6d 5氮胞苷与丁酸处理组的HepG2细胞数分别为 (13 2± 0 6 )× 10 5和 (6 1± 0 6 )× 10 5,明显少于对照组(2 1 6± 1 0 )× 10 5;Hep3B细胞数分别为 (13 7± 1 1)× 10 5和 (4 6± 0 7)× 10 5,明显少于对照组的(18 0± 1 6 )× 10 5,P <0 0 1。附壁试验显示接种后 12 0min ,5氮胞苷与丁酸处理组HepG2附壁细胞百分比分别为 5 9 6± 2 6和 41 8± 2 1,明显少于对照组... 【英文摘要】 Objective We have recently shown that 5-azacytidine and butyrate can trigger apoptosis in human liver cancer cells. The purpose of our present study was to determine the effects of 5-azacytidine and butyrate on the invasion of liver cancer cells and the changes of the protein expression in the liver cancer cells during the treatment with these agents. Method Human liver cancer cell lines HepG2 and Hep3B were treated with either 5-azacytidine or butyrate; in vitro cell invasion, adhesion and proliferation...