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dc.contributor.advisor张鹏
dc.contributor.author陈美琼
dc.date.accessioned2016-01-13T09:10:46Z
dc.date.available2016-01-13T09:10:46Z
dc.date.issued2014-02-27 15:31:15.0
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/79683
dc.description.abstract目的:通过RNA干扰技术,靶向沉默人多发性骨髓瘤RPMI8226细胞Notch1基因,评估Notch1表达下调后对多发性骨髓瘤细胞增殖、凋亡,相关蛋白Hes-1、Jagged-1、 Jagged-2及Bcl-2、PTEN/PI3K/AKT信号通路的影响,进一步探讨Notch1在多发性骨髓瘤发生发展中的作用及分子机制,为寻找新的骨髓瘤治疗靶点提供实验依据。 方法:构建靶向沉默Notch1基因的shRNA表达载体pGshRNA-Notch1,转染入人多发性骨髓瘤RPMI8226细胞,G418筛选稳定转染细胞。实时荧光定量PCR及Westernblot法检测转染后Notch1mRNA和蛋白表达的...
dc.description.abstractObjective: To illustrate the mechanisms of Notch1 gene silence on cell proliferation, apoptosis and PTEN/PI3K/AKT signaling pathway of multiple myeloma RPMI8226 cells, and therefore provide the evidences for novel targeted therapeutic strategy for MM. Materials and Methods: Notch1 short hairpin RNA(Notch1-shRNA) was transfected into RPMI 8226 cells and selected by G418. Real-time quantitative PCR...
dc.language.isozh_CN
dc.relation.urihttp://210.34.4.28/opac/openlink.php?strText=40572&doctype=ALL&strSearchType=callno
dc.source.urihttp://210.34.4.13:8080/lunwen/detail.asp?serial=41187
dc.subject多发性骨髓瘤
dc.subjectshRNA
dc.subjectNotch信号
dc.subjectPTEN/AKT信号
dc.subjectMultiple Myeloma
dc.subjectshRNA
dc.subjectNotch
dc.subjectPI3K/AKT
dc.titleRNAi沉默Notch1基因对多发性骨髓瘤细胞致瘤性影响及机制研究
dc.title.alternativeEffects and mechanisms of Notch1 gene silencing by RNA interference on oncogenicity of human Multiple Myeloma Cells
dc.typethesis
dc.date.replied2013-05-24
dc.description.note学位:医学硕士
dc.description.note院系专业:医学院_内科学
dc.description.note学号:24520101153272


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