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dc.contributor.authorZhang, H. Z.zh_CN
dc.contributor.authorLi, X. M.zh_CN
dc.contributor.authorGao, F. P.zh_CN
dc.contributor.authorLiu, L. R.zh_CN
dc.contributor.authorZhou, Z. M.zh_CN
dc.contributor.authorZhang, Q. Q.zh_CN
dc.contributor.author张其清zh_CN
dc.date.accessioned2013-12-12T02:51:18Z
dc.date.available2013-12-12T02:51:18Z
dc.date.issued2010-01zh_CN
dc.identifier.citationDrug Delivery, 2010,17(1):48-57zh_CN
dc.identifier.issn1071-7544zh_CN
dc.identifier.otherISI:000274722800006zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/71452
dc.descriptionMajor State Basic Research Program of China [933300]; Doctoral Fund of Ministry of Education of China [2006023050]zh_CN
dc.description.abstractThe purpose of this work was to develop a novel nano-carrier with targeting property to tumor. In this study, pullulan acetate (PA) was synthesized by the acetylation of pullulan to simplify the preparation technique of nanoparticles. Folic acid (FA) was conjugated to PA in order to improve the cancer-targeting activity. The products were characterized by proton nuclear magnetic resonance ((1)H NMR) spectroscopy. Epirubicin-loaded nanoparticles were prepared by a solvent diffusion method. The loading efficiencies and EPI content increased with the amount of triethylamine (TEA) increasing in some degree. FPA nanoparticles could incorporate more epirubicin than PA nanoparticles. The folate-modifed PA nanoparticles (FPA/EPI NPs) exhibited faster drug release than PA nanoparticles (PA/EPI NPs) in vitro. Confocal image analysis and flow cytometry test revealed that FPA/EPI NPs exhibited a greater extent of cellular uptake than PA/EPI NPs against KB cells over-expressing folate receptors on the surface. FPA/EPI NPs also showed higher cytotoxicity than PA/EPI NPs. The cytotoxic effect of FPA/EPI NPs to KB cells was inhibited by an excess amount of folic acid, suggesting that the binding and/or uptake were mediated by the folate receptor.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.3109/10717540903508979zh_CN
dc.subjectASSEMBLED HYDROGEL NANOPARTICLESzh_CN
dc.subjectSOLVENT DIFFUSION METHODzh_CN
dc.subjectHYDROPHOBIZED PULLULANzh_CN
dc.subjectBINDING-PROTEINzh_CN
dc.subjectCELL-LINESzh_CN
dc.subjectIN-VITROzh_CN
dc.subjectRECEPTORzh_CN
dc.subjectCONJUGATEzh_CN
dc.subjectDOXORUBICINzh_CN
dc.subjectPHzh_CN
dc.titlePreparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug deliveryzh_CN
dc.typeArticlezh_CN


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