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Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery

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Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery.pdf (1.175Mb)
Date
2010-01
Author
Zhang, H. Z.
Li, X. M.
Gao, F. P.
Liu, L. R.
Zhou, Z. M.
Zhang, Q. Q.
张其清
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  • 医学院-已发表论文 [2663]
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Abstract
The purpose of this work was to develop a novel nano-carrier with targeting property to tumor. In this study, pullulan acetate (PA) was synthesized by the acetylation of pullulan to simplify the preparation technique of nanoparticles. Folic acid (FA) was conjugated to PA in order to improve the cancer-targeting activity. The products were characterized by proton nuclear magnetic resonance ((1)H NMR) spectroscopy. Epirubicin-loaded nanoparticles were prepared by a solvent diffusion method. The loading efficiencies and EPI content increased with the amount of triethylamine (TEA) increasing in some degree. FPA nanoparticles could incorporate more epirubicin than PA nanoparticles. The folate-modifed PA nanoparticles (FPA/EPI NPs) exhibited faster drug release than PA nanoparticles (PA/EPI NPs) in vitro. Confocal image analysis and flow cytometry test revealed that FPA/EPI NPs exhibited a greater extent of cellular uptake than PA/EPI NPs against KB cells over-expressing folate receptors on the surface. FPA/EPI NPs also showed higher cytotoxicity than PA/EPI NPs. The cytotoxic effect of FPA/EPI NPs to KB cells was inhibited by an excess amount of folic acid, suggesting that the binding and/or uptake were mediated by the folate receptor.
Description
Major State Basic Research Program of China [933300]; Doctoral Fund of Ministry of Education of China [2006023050]
Citation
Drug Delivery, 2010,17(1):48-57
URI
https://dspace.xmu.edu.cn/handle/2288/71452

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