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dc.contributor.authorZhang, Zhubaozh_CN
dc.contributor.authorLuo, Qiangzh_CN
dc.contributor.authorYan, Xiaowenzh_CN
dc.contributor.authorLi, Zhaoxinzh_CN
dc.contributor.authorLuo, Yacuizh_CN
dc.contributor.authorYang, Liminzh_CN
dc.contributor.authorZhang, Bozh_CN
dc.contributor.authorChen, Haifengzh_CN
dc.contributor.authorWang, Qiuquanzh_CN
dc.contributor.author陈海峰zh_CN
dc.date.accessioned2013-12-12T02:25:47Z
dc.date.available2013-12-12T02:25:47Z
dc.date.issued2012-11-06zh_CN
dc.identifier.citationAnalytical Chemistry, 2012,84(21):8946-8951zh_CN
dc.identifier.issn0003-2700zh_CN
dc.identifier.otherWOS:000310664600009zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/66048
dc.descriptionNational Natural Science Foundation of China [21035006, 21275120]; National Basic Research 973 Program [2009CB421605]zh_CN
dc.description.abstractWe report the design and synthesis of a trifunctional probe for seeing and counting cancer cells using both fluorescence imaging (FI) and inductively coupled plasma mass spectrometry (ICPMS) for the first time. It consisted of a guiding cyclic RGD peptide unit to catch cancer cells via targeting the alpha(v)beta(3) integrin overexpressed on their surface, a 5-amino-fluorescein moiety for FI using confocal laser scanning microscopy (CLSM) as well as a 2-aminoethyl-monoamide-DOTA group for loading stable europium ion and subsequent ICPMS quantification of the cancer cells without the use of radioactive isotopes. In addition to FI, the LOD (3 sigma) of the alpha(v)beta(3) integrin was down to 69.2-309.4 amol per cell depending on the type of the alpha(v)beta(3)-positive cancer cells when using ICPMS and those of the cancer cell number reached 17-75. This probe developed enables us not only to see but also to count the alpha(v)beta(3)-positive cancer cells ultrasensitively, paving a new way for early diagnosis of cancer.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1021/ac302029wzh_CN
dc.subjectQUANTUM DOTSzh_CN
dc.subjectBREAST-CANCERzh_CN
dc.subjectALPHA(V)BETA(3)zh_CN
dc.subjectQUANTIFICATIONzh_CN
dc.subjectALPHA-V-BETA-3zh_CN
dc.subjectFLUORESCENCEzh_CN
dc.subjectFIBRONECTINzh_CN
dc.subjectTOMOGRAPHYzh_CN
dc.subjectEXPRESSIONzh_CN
dc.subjectLIGANDzh_CN
dc.titleIntegrin-Targeted Trifunctional Probe for Cancer Cells: A 'Seeing and Counting' Approachzh_CN
dc.typeArticlezh_CN


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